Reference : Rôle des gènes pax4 et arx au cours de la différenciation endocrine pancréatique chez...
Dissertations and theses : Doctoral thesis
Life sciences : Biochemistry, biophysics & molecular biology
Rôle des gènes pax4 et arx au cours de la différenciation endocrine pancréatique chez le poisson zèbre
[en] Role of pax4 and arx gene during pancreatic endocrine cell development
Djiotsa, Joachim mailto [Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Université de Liège, ​Liège, ​​Belgique
Docteur en Sciences
Voz, Marianne mailto
Manfroid, Isabelle mailto
Piette, Jacques mailto
Geenen, Vincent mailto
Grobet, Luc mailto
Munaut, Carine mailto
JACQUEMIN, Patrick mailto
[en] pax4 ; pancreas ; arx ; gene ; mRNA export ; insulin ; glucagon ; development ; zebrafish
[en] Genetic studies in mouse have demonstrated the crucial function of PAX4 in pancreatic cell differentiation. This transcription factor specifies β- and δ-cell fate at the expense of α-cell identity by repressing Arx gene expression and ectopic expression of PAX4 in α-cells is sufficient to convert them into β-cells. Surprisingly, no Pax4 orthologous gene can be found in chicken and xenopus tropicalis raising the question of the function of Pax4 gene in lower vertebrates such as in fish. In the present study, we have analysed the expression and the function of the orthologous pax4 gene in zebrafish. In zebrafish, pax4 is not required for the generation of pancreatic β- and δ-cells, unlike its crucial role in the differentiation of these cell types in mouse. On the other hand, the mutual repression between Arx and Pax4 is observed in both mouse and zebrafish. These data suggests that the main original function of Pax4 during vertebrate evolution was to modulate the number of pancreatic α-cells and its role in β-cells differentiation appeared later in vertebrate evolution.
GIGA-research, LBMGG
poisson zèbre
pancreas development
Researchers ; Professionals ; Students

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