Article (Scientific journals)
Assessment of erythromycin toxicity on activated sludge via batch experiments and microscopic techniques (epifluorescence and CLSM)
Louvet, Jean-Noël; Helouin, Yannick; Attik, Ghania et al.
2010In Process Biochemistry, 45, p. 1187-1794
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Keywords :
Macrolides; Antibiotic; Inhibition; BacLight; Microscopy
Abstract :
[en] This study investigates erythromycin toxicity toward activated sludge as a function of exposure time and antibiotic concentration. Batch experiments were conducted and microscopic techniques ranging from bright-field microscopy to epifluorescence and confocal laser scanning microscopy (CLSM), combined with a fluorescent viability indicator (BacLight ® Bacterial Viability Kit, Molecular Probes), allowed us to study erythromycin time-kill activity. The erythromycin toxicity was observed at lower concentration when exposure time increased. A 4 .g/L erythromycin concentration was toxic to heterotrophic bacteria on a 5-day time exposure, and a 5 mg/L concentration inhibited nitrification. These findings are in agreement with the microscopic studies, which showed a latency time before the lower antibiotic concentrations began to kill bacteria. Microscope slide wells were used as micro-reactors in which erythromycin concentration ranged from 0.1 to 1 mg/L. After 45 min there were 94% (SD 3.8) of living bacteria in control micro-reactors, 67% (SD 3.1) in micro-reactors that contained 0.1 mg/L erythromycin and 37% (SD 18.6) in micro-reactors that contained 1 mg/L erythromycin. CLSM allowed visualization of isolated stained cells in the three-dimensional structure of damaged flocs
Research center :
CNRS
Disciplines :
Chemical engineering
Author, co-author :
Louvet, Jean-Noël ;  LRGP (UPR 3349 CNRS) Nancy Université INPL
Helouin, Yannick;  LRGP (UPR 3349 CNRS) Nancy Université INPL
Attik, Ghania;  LRGP (UPR 3349 CNRS) Nancy Université INPL
Dumas, Dominique;  Nancy-Université, Imaging Facility, FR 3209, 7561 CNRS, Faculty of Medicine,
Potier, Olivier;  LRGP (UPR 3349 CNRS) Nancy Université INPL
Pons, Marie-Noëlle
Language :
English
Title :
Assessment of erythromycin toxicity on activated sludge via batch experiments and microscopic techniques (epifluorescence and CLSM)
Publication date :
2010
Journal title :
Process Biochemistry
ISSN :
1359-5113
Publisher :
Elsevier Science, Oxford, United Kingdom
Volume :
45
Pages :
1187-1794
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
ANR - Agence Nationale de la Recherche [FR]
Available on ORBi :
since 17 June 2012

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