Available on ORBi since
18 April 2010
Article (Scientific journals)
Immunocytochemical evidence for production of luteinizing hormone and follicle-stimulating hormone in separate cells in the bovine.
Bastings, E.; Beckers, Albert  ; Reznik, Michel  et al.
1991 • In Biology of Reproduction, 45 (5), p. 788-96
Peer Reviewed verified by ORBi
 

Files


Full Text
Immunocytochemical evidence for production of luteinizing hormone and follicle-stimulating hormone in separate cells in the bovine..pdf
Publisher postprint (1.71 MB)

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Animals; Cattle; Cytoplasmic Granules/chemistry; Female; Follicle Stimulating Hormone/analysis/biosynthesis; Immunohistochemistry; Luteinizing Hormone/analysis/biosynthesis; Male; Microscopy, Electron; Pituitary Gland/chemistry/ultrastructure
Abstract :
[en] In all mammalian females, follicular growth and maturation are essentially dependent on the pituitary gonadotropins, FSH and LH. These glycoprotein hormones have many similarities, but their action, based on high affinity binding to specific membrane receptors, are quite different. The purpose of this study was to perform a sensitive localization of FSH and LH in secretory granules of gonadotrophs using highly specific antisera. This morphological study included light microscopy (PAP) and electron microscopy (immunogold single and double labeling) procedures. Histologically, approximatively 11.5% of cells were positive for LH, whereas only 5.4% of cells were positive for FSH. With the electron microscope, single labeling allowed identification of morphologically distinct LH-containing cells and FSH-containing cells. Double immunostaining confirmed that no cells contained both hormones. The finding that FSH and LH are produced in separate pituitary cells is in agreement with recent studies that have suggested a specific role and regulatory process for gonadotropins in the bovine species.
Research center :
Laboratoire d'Endocrinologie et de Physiologie de la Reproduction animale
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Bastings, E.
Beckers, Albert ;  Université de Liège - ULiège > Département des sciences cliniques > Endocrinologie
Reznik, Michel ;  Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Beckers, Jean-François ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Immunocytochemical evidence for production of luteinizing hormone and follicle-stimulating hormone in separate cells in the bovine.
Publication date :
1991
Journal title :
Biology of Reproduction
ISSN :
0006-3363
eISSN :
1529-7268
Publisher :
Society for the Study of Reproduction, Madison, United States - Wisconsin
Volume :
45
Issue :
5
Pages :
788-96
Peer reviewed :
Peer Reviewed verified by ORBi

Statistics


Number of views
45 (4 by ULiège)
Number of downloads
135 (3 by ULiège)

Scopus citations®
 
24
Scopus citations®
without self-citations
24
OpenCitations
 
14

Bibliography


Similar publications



Contact ORBi