Sperm quality analysis in XX, XY and YY males of the Nile tilapia (Oreochromis niloticus).

[en] In Nile tilapia (Oreochromis niloticus), individuals with atypical sexual genotype are commonly used in farming (use of YY males to produce all-male offsprings), but they also constitute major tools to study sex determinism mechanisms. In other species, sexual genotype and sex reversal procedures affect different aspects of biology such as growth, behaviour and reproductive success. The aim of this study was to assess the influence of sexual genotype on sperm quality in Nile tilapia. Milt characteristics were compared in XX (sex-reversed), XY and YY males in terms of gonadosomatic index, sperm count, sperm motility and duration of sperm motility. Sperm motility was measured by computer-assisted sperm analysis (CASA) quantifying several parameters: total motility, progressive motility, curvilinear velocity, straight line velocity, average path velocity and linearity. None of the sperm trait measured differed significantly between the three genotypes. Mean values of gonadosomatic index, sperm concentration and sperm motility duration of XX, XY and YY males respectively ranged from 0.92 to 1.33 %, from 1.69 to 2.22 × 10(9) cells mL-1 and from 18’04’’ to 27’32’’. Mean values of total motility and curvilinear velocity 1 min after sperm activation respectively ranged from 53 to 58 % and from 71 to 76 µm s-1 for the three genotypes. After 3 min of activity, all the sperm motility and velocity parameters dropped by half and continued to slowly decrease thereafter. Seven min after activation, only 9 to 13 % of spermatozoa were still progressive. Our results prove that neither sexual genotype nor hormonal sex reversal treatments affect sperm quality in male Nile tilapias with atypical sexual genotype.

Research Center/Unit :

AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège

Disciplines :

Zoology

Author, co-author :

Gennotte, Vincent ; Université de Liège - ULiège > Centre de formation et de recherche en aquaculture (CEFRA)

François, Ekniel; Caribbean Harvest, Impasse Dessources 2, Vivy Mitchel-Petion Ville, Haiti

Rougeot, Carole ; Université de Liège - ULiège > Centre de formation et de recherche en aquaculture (CEFRA)

Ponthier, Jérôme ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux

Deleuze, Stefan ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Obstét. et path. de la reprod. des anim. de comp. et équidés

Mélard, Charles ; Université de Liège - ULiège > Centre de formation et de recherche en aquaculture (CEFRA)

Language :

English

Title :

Sperm quality analysis in XX, XY and YY males of the Nile tilapia (Oreochromis niloticus).

Publication date :

2012

Journal title :

Theriogenology

ISSN :

0093-691X

eISSN :

1879-3231

Publisher :

Elsevier, New York, United States - New York

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://dx.doi.org/10.1016/j.theriogenology.2012.02.002

Commentary :

Available on ORBi :

since 03 June 2012

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