Differential accumulation of mRNA for immediate early genes and heat shock genes in heart after ischaemic injury.

Animals; Carrier Proteins/biosynthesis; Coronary Vessels; Creatine Kinase/blood; DNA-Binding Proteins/biosynthesis; Early Growth Response Protein 1; Genes, Immediate-Early; Genes, fos; Genes, jun; HSC70 Heat-Shock Proteins; HSP70 Heat-Shock Proteins/biosynthesis; Heart/physiology/physiopathology; Heat-Shock Proteins/biosynthesis; Hot Temperature; Immediate-Early Proteins; In Situ Hybridization; Kinetics; Male; Myocardial Contraction; Myocardial Ischemia/metabolism/pathology/physiopathology; Myocardium/metabolism/pathology; Necrosis; Oligonucleotide Probes; RNA, Messenger/biosynthesis; Rats; Rats, Sprague-Dawley; Retroviridae Proteins, Oncogenic/biosynthesis; Time Factors; Transcription Factors; Transcription, Genetic

Abstract :

[en] Ischaemic injury leads to the expression of heat shock and immediate early genes. Here the localization of this induction is examined by in situ hybridization analysis in serial sections of buffer-perfused isolated rat heart after 30 min of coronary artery occlusion. The accumulation of mRNA for hsc70, hsp70, c-fos, c-jun, and Erg-1 was localized coincidently and was restricted to the ischaemic area of the heart. mRNA for these genes was undetectable at the end of the ischaemic period (no reperfusion). After 30 min of reperfusion, accumulation of mRNA for hsc70, hsp70, c-fos, and c-jun was detectable and increased with further reperfusion. Within the area labelled for these gene products was a central area of less intense labelling which corresponded to the necrotic zone. The immediate early gene product, jun-B, was localized in both the ischaemic and the non-ischaemic area of the hearts. These results suggest that the area of the heart where heat shock and immediate early gene transcripts accumulate, while injured, recovers transcriptional activity, and that the central area where minimal heat shock and immediate early gene transcripts accumulate, does not recover transcriptional activity and is irreversibly injured.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Plumier, Jean-Christophe ; Dalhousie University > Anatomy and Neurobiology

Robertson, H. A.; Dalhousie University > Pharmacology

Currie, R. W.; Dalhousie University > Anatomy and Neurobiology

Language :

English

Title :

Differential accumulation of mRNA for immediate early genes and heat shock genes in heart after ischaemic injury.

Publication date :

1996

Journal title :

Journal of Molecular and Cellular Cardiology

ISSN :

0022-2828

eISSN :

1095-8584

Publisher :

Academic Press, London, United Kingdom

Volume :

Issue :

Pages :

1251-60

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 06 May 2009

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