Differential expression of c-fos, Hsp70 and Hsp27 after photothrombotic injury in the rat brain.

Animals; Brain Injuries/metabolism/pathology; Cerebral Cortex/metabolism/pathology; Fiber Optic Technology; Frontal Lobe/injuries; Gene Expression; HSP70 Heat-Shock Proteins/biosynthesis; Heat-Shock Proteins/biosynthesis; Intracranial Embolism and Thrombosis/metabolism/pathology; Male; Necrosis; Parietal Lobe/injuries; Protein Biosynthesis; Proto-Oncogene Proteins c-fos/biosynthesis; RNA, Messenger/biosynthesis; Rats; Rats, Inbred Strains; Transcription, Genetic

Abstract :

[en] In situ hybridization and immunohistochemistry were used to examine the expression of c-fos, Hsp70 and Hsp27 following photothrombotic injury in the right fronto-parietal cortex of the rat. C-fos mRNA and protein were detected in the entire cerebral cortex on the lesioned side. Hsp70 mRNA accumulation was observed only adjacent and peripheral to the site of the lesion. At 1 h after photothrombotic injury, Hsp70 expression delineates the area of necrosis at 24 h after photothrombotic injury. Hsp27 protein was observed in the ipsilateral cerebral cortex with the exception of the deep layers of the cingulate cortex. In addition, while c-Fos immunoreactivity was localized in cell nuclei, Hsp27 immunoreactivity was detected in the cytoplasm of astrocytes. These results demonstrate that unilateral cortical injury induces changes in gene expression that vary according to cell type and brain region.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Plumier, Jean-Christophe ; Dalhousie University > Anatomy and Neurobiology

Armstrong, J. N.; Dalhousie University > Pharmacology, Anatomy and Neurobiology

Wood, N. I.; SmithKline Beecham Pharmaceuticals

Babity, J. M.; Dalhousie University > Pharmacology, Anatomy and Neurobiology

Hamilton, T. C.; SmithKline Beecham Pharmaceuticals

Hunter, A. J.; SmithKline Beecham Pharmaceuticals

Robertson, H. A.; Dalhousie University > Pharmacology

Currie, R. W.; Dalhousie University > Anatomy and Neurobiology

Language :

English

Title :

Differential expression of c-fos, Hsp70 and Hsp27 after photothrombotic injury in the rat brain.

Publication date :

1997

Journal title :

Molecular Brain Research

ISSN :

0169-328X

Publisher :

Elsevier Science

Volume :

Issue :

Pages :

239-46

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 06 May 2009

Statistics

Number of views

49 (4 by ULiège)

Number of downloads

1 (0 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Armstrong, J.N., Plumier, J.-C.L., Robertson, H.A. and Currie, R.W., The inducible 70,000 mol. wt heat shock protein is expressed in degenerating dentate hilus and piriform cortex after systemic administration of kainic acid in the rat, Neuroscience, 74 (1996) 685-693.
Brown, I.R., Rush, S. and Ivy, G.O., Induction of a heat shock gene at the site of tissue injury in the rat brain, Neuron, 2 (1989) 1559-1564.
Comelli, M.C., Guidolin, D., Seren, M.S., Zanoni, R., Canella, R., Rubini, R. and Manev, H, Time course, localization and pharmacological modulation of immediate early inducible genes, brain-derived neurotrophic factor and trkB messenger RNAs in the rat brain following photochemical stroke, Neuroscience, 55 (1993) 473-490.
Currie, R.W. and White, F.P., Trauma-induced protein in rat tissues: A physiological role for a 'heat shock' protein? Science, 214 (1981) 72-73.
Curran, T. and Morgan, J.I., Fos: An immediate-early transcription factor in neurons, J. Neurobiol., 26 (1995) 403-412.
David, J.-C., Currie, R.W. and Robertson, H.A., Expression and distribution of Hsp70 and hsc73 messenger RNAs in rat brain following heat shock: Effect of dizocilpine maleate, Neuroscience, 62 (1994) 945-954.
Dragunow, M. and Robertson, H.A., Brain injury induces c-fos protein(s) in nerve and glial-like cells in adult mammalian brain, Brain Res., 455 (1988) 295-299.
Gass, P., Prior, P. and Kiessling, M., Correlation between seizure intensity and stress protein expression after limbic epilepsy in the rat brain, Neuroscience, 65 (1995) 27-36.
Ginsberg, S.D., Martin, L.J. and Rothstein, J.D., Regional deafferentation down-regulates subtypes of glutamate transporter proteins, J. Neurochem., 65 (1995) 2800-2803.
Gonzalez, M.F., Lowenstein, D., Fernyak, S., Hisanaga, K., Simon, R. and Sharp, F.R., Induction of heat shock protein 72-like immunoreactivity in the hippocampal formation following transient global ischemia, Brain Res. Bull., 26 (1991) 241-250.
Gonzalez, M.F., Shiraishi, K., Kisanaga, K., Sagar, S.M., Mandabach, M. and Sharp, F.R., Heat shock proteins as markers of neural injury, Mol. Brain Res., 6 (1989) 93-100.
Herrera, D.G. and Cuello, A.C., MK-801 affects the potassium-induced increase of glial fibrillary acidic protein immunoreactivity in rat brain, Brain Res., 598 (1992) 286-293.
Herrera, D.G. and Robertson, H.A., Unilateral induction of c-fos protein in cortex following cortical devascularization, Brain Res., 503 (1989) 205-213.
Herrera, D.G. and Robertson, H.A., Application of potassium chloride to the brain surface induces the c-fos proto-oncogene: Reversal by MK-801, Brain Res., 510 (1990) 166-170.
Iwaki, T., Iwaki, A., Tateishi, J., Sakaki, Y. and Goldman, J.E., αB-crystallin and 27-kD heat shock protein are regulated by stress conditions in the central nervous system and accumulate in Rosenthal fibers, Am. J. Pathol., 143 (1993) 487-495.
Jacobs, O., Van Bree, L., Mailleux, P., Zhang, F., Schiffmann, S.N., Halleux, P., Albala, N. and Vanderhaeghen, J.-J., Homolateral cerebrocortical increase of immediate early gene and neurotransmitter messenger RNAs after minimal cortical lesion: Blockade by N-methyl-D-aspartate antagonist, Neuroscience, 59 (1994) 827-836.
Kato, H., Liu, Y., Kogure, K. and Kato, K., Induction of 27-kDa heat shock protein following cerebral ischemia in a model of ischemic tolerance, Brain Res., 634 (1994) 235-244.
Kato, H., Kogure, K., Liu, X.H., Araki, T., Kato, K. and Itoyama, Y., Immunohostochemical localization of the low molecular weight stress protein Hsp27 following focal cerebral ischemia in the rat, Brain Res., 679 (1995) 1-7.
Kinouchi, H., Sharp, F.R., Koistinaho, J., Hicks, K., Kamii, H. and Chan, P.H., Induction of heat shock Hsp70 mRNA and HSP70 kDa protein in neurons in the 'penumbra' following focal cerebral ischemia in the rat, Brain Res., 619 (1993) 334-338.
Kogure, K. and Kato, H., Altered gene expression in cerebral ischemia, Stroke, 24 (1993) 2121-2127.
Landry, J., Chrétien, P., Lambert, H., Hickey, E. and Weber, L.A., Heat shock resistance conferred by expression of the human HSP27 gene in rodent cells, J. Cell Biol., 109 (1989) 7-15.
Lavoie, J.N., Hickey, E., Weber, L.A. and Landry, J., Modulation of actin microfilament dynamics and fluid phase pinocytosis by phosphorylation of heat shock protein 27, J. Biol. Chem., 268 (1993) 24210-24214.
Lindsberg, P.J., Frerichs, K.U., Sirén, A.-L., Hallenbeck, J.M. and Nowak, T.S. Jr., Heat-shock protein and c-fos expression in focal microvascular brain damage, J. Cereb. Blood Flow Metab., 16 (1996) 82-91.
Lowenstein, D.H., Simon, R.P. and Sharp, F.R., The pattern of 72-kDa heat shock protein-like immunoreactivity in the rat brain following flurothyl-induced status epilepticus, Brain Res., 531 (1990) 173-182.
Marber, M.S., Mestri, R., Chi, S.H., Sayen, M.R., Yellon, D.M. and Dillmann, W.H., Overexpression of the rat inducible 70-kD heat stress protein in a transgenic mouse increases the resistance of the heart to ischemic injury, J. Clin. Invest., 95 (1995) 1446-1456.
Mestril, R., Chi, S.-H., Sayen, M.R., O'Reilly, K. and Dillmann, W.H., Expression of inducible stress protein 70 in rat heart myogenic cells confers protection against stimulated ischemia-induced injury, J. Clin. Invest., 93 (1994) 759-767.
Plumier, J.-C.L., Robertson, H.A. and Currie, R.W., Differential accumulation of mRNA for immediate early genes and heat shock genes in heart after ischemic injury, J. Mol. Cell. Cardiol., 28 (1996) 1251-1260.
Plumier, J.-C.L., Armstrong, J.N., Landry, J., Robertson, H.A. and Currie, R.W., Expression of the 27,000 mol. wt heat shock protein following kainic acid-induced status epilepticus in the rat, Neuroscience, (1996) in press.
Plumier, J.-C.L., Ross, B.M., Currie, R.W., Angelidis, C.E., Kazlaris, H., Kollias, G. and Pagoulatos, G.N., Transgenic mice expressing the human heat shock protein 70 have improved post-ischemic myocardial recovery, J. Clin. Invest., 95 (1995) 1854-1860.
Sharp, F.R., Lowenstein, D., Simon, R. and Hisanaga, K., Heat shock protein Hsp72 induction in cortical and striatal astrocytes and neurons following infarction, J. Cereb. Blood Flow Metab., 11 (1991) 621-627.
Simon, R.P., Cho, H., Gwinn, R. and Lowenstein, D.H., The temporal profile of 72-kDa heat-shock protein expression following global ischemia, J. Neurosci., 11 (1991) 881-889.
Sistonen, L., Sarge, K.D. and Morimoto, R.I., Human heat shock factors 1 and 2 are differentially activated and can synergistically induce HSP70 gene transcription, Mol. Cell Biol., 14 (1994) 2087-2099.
Vass, K., Berger, M.L., Nowak, T.S., Jr., Welch, W.J. and Lassmann, H., Induction of stress protein HSP70 in nerve cells after status epilepticus in the rat, Neurosci. Lett., 100 (1989) 259-264.
Williams, R.S., Thomas, J.A., Fina, M., German, Z. and Benjamin, I.J., Human heat shock protein 70 (Hsp70) protects murine cells from injury during metabolic stress, J. Clin. Invest., 92 (1993) 503-508.
Winter, C.G., Saotome, Y., Levison, S.W. and Hirsh, D., A role for ciliary neurotrophic factor as an inducer of reactive gliosis, the glial response to central nervous system injury, Proc. Natl. Acad. Sci. USA, 92 (1995) 5865-5869.