[en] BACKGROUND: Within semi-closed areas like the Mediterranean Sea, anthropic wastes tend to concentrate in the environment. Metals, in particular, are known to persist in the environment and can affect human health due to accumulation in the food chain. The seagrass Posidonia oceanica, widely found in Mediterranean coastal waters, has been chosen as a "sentinel" to quantify the distribution of such pollutants within the marine environment. Using a technique similar to dendrochronology in trees, it can act as an indicator of pollutant levels over a timeframe of several months to years. In the present study, we measured and compared the levels of eight trace metals (Cr, Ni, Cu, Zn, As, Se, Cd, and Pb) in sheaths dated by lepidochronology and in leaves of shoots sampled from P. oceanica meadows collected from six offshore sites in northern Corsica between 1988 and 2004; in the aim to determine 1) the spatial and 2) temporal variations of these metals in these areas and 3) to compared these two types of tissues. RESULTS: We found low trace metal concentrations with no increase over the last decade, confirming the potential use of Corsican seagrass beds as reference sites for the Mediterranean Sea. Temporal trends of trace metal concentrations in sheaths were not significant for Cr, Ni, Cu, As or Se, but Zn, Cd, and Pb levels decreased, probably due to the reduced anthropic use of these metals. Similar temporal trends between Cu levels in leaves (living tissue) and in sheaths (dead tissue) demonstrated that lepidochronology linked with Cu monitoring is effective for surveying the temporal variability of this metal. CONCLUSION: Leaves of P. oceanica can give an indication of the metal concentration in the environment over a short time period (months) with good accuracy. On the contrary, sheaths, which gave an indication of changes over long time periods (decades), seem to be less sensitive to variations in the metal concentration in the environment. Changes in human consumption of metals (e.g., the reduction of Pb in fuel) are clearly reflected in both organs. These results confirm that P. oceanica is a good bioindicator of metals and a good biomonitor species for assessing Cu in the environment.
Research center :
MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège
El Sayed MA, Aminot A, Kerouel R: Nutrients and trace metals in the North-western Mediterranean under coastal upwelling conditions. Continental Shelf Research 1994, 14:507-530.
Copin-Montégut G, Courceau P, Nicholas E: Distribution and transfer of trace elements in the Western Mediterranean. Marine Chemistry 1986, 18:189-195.
Ramade F: Conservation des Ecosystèmes méditerrané ens - Enjeux et Perspectives. Les Fascicules du Plan Bleu, PNUE/PAM 1990, 3:1-144.
Péres JM, Picard J: Causes de la raréfaction et de la disparition des herbiers de Posidonia oceanica sur les côtes françaises de la Méditerranée. Aquatic Botany 1975, 1:133-139.
Meinesz A, Lefevre JR, Astier JM: Impact of coastal development on the infralittoral zone along the southern Mediterranean shore of continental France. Marine Pollution Bulletin 1991, 23:343-347.
Pergent G, Pergent-Martini C, Boudouresque CF: Utilisation de l'herbier à Posidonia oceanica comme indicateur biologique de la qualité du milieu littoral en Méditerranée: état des connaissances. Mésogée 1995, 54:3-27.
Guidetti P, Fanelli G, Frachetti S, Terlizzi A, Boero F: Coastal fish indicate human-induce changes in the Mediterranean littoral. Marine Environmental Research 2002, 53:77-94.
Piazzi L, Cinelli F: Effects de l'expansion des Rhodophyceae introduites Acrothamnion preissii et Womersleyla setacea sur les communautés algales des rhizomes de Posidonia oceanica de Méditerranée occidentale. Cryptogamie Algologie 2000, 21:291-300.
Boudouresque CF, Verlaque M: Biological pollution in the Mediterranean Sea: invasive versus introduced macrophytes. Marine Pollution Bulletin 2002, 44:32-38.
Argyrou M, Demetropoulos A, Hadjichristophorou M: Expansion of the macroalga Caulerpa racemosa and changes in softbottom macrofaunal assemblages in Moni Bay, Cyprus. Oceanologica Acta 1999, 22:517-528.
Ceccherelli G, Cinelli F: Effects of Posidonia oceanica canopy on Caulerpa taxifolia size in a north-western Mediterranean bay. Journal of Experimental Marine Biology and Ecology 1999, 240:19-36.
Livre rouge «Gérard Vuignier» des végé taux, peuplement et paysages marins menacés de Méditerrané e. UNEP/IUCN/GIS Posidonie, MAP Technical Report Series 1990.
Platini F: La protection des habitats aux herbiers en Méditerranée. Report. PNUE, PAM, CAR/ASP 2000.
Markert BA, Breure AM, Zechmeister HG: Bioindication/biomonitoring of the environment. Bioindicators and Biomonitors principles, concepts and applications. Trace metals and other contaminants in the environment 2003, 6:3-40.
Pergent-Martini C, Pergent G: Marine phanerogams as a tool in the evaluation of marine trace-metal contamination: an example from the Mediterranean. International Journal Environment and Pollution 2000, 13:126-147.
Ferrat L, Pergent-Martini C, Roméo M: Assessment of the use of biomarkers in aquatic plant for the evaluation of environmental quality: application to seagrasses. Aquatic Toxicology 2003, 65:187-204.
Pergent-Martini C, Leoni V, Pasqualini V, Ardizzone GD, Balestri E, Bedini R, Belluscio A, Belsher T, Borg J, Boudouresque CF, Boumaza S, Bouquegneau JM, Buia MC, Calvo S, Cebrian J, Charbonnel E, Cinelli F, Cossu A, Di Maida G, Dural B, Francour P, Gobert S, Lepoint G, Meinesz A, Molenaar H, Mansour HM, Panayotidis P, Peirano A, Pergent G, Piazzi L, Pirrotta M, Renili G, Romero J, Sanchez-Lizaso JL, Semroud R, Shembri P, Shili A, Tomasello A, Velmirov B: Descriptors of Posidonia oceanica meadows: use and application. Ecological Indicators 2005, 5:213-230.
Pergent G, Boudouresque CF, Crouzet A, Meinesz A: Cyclic changes along Posidonia oceanica rhizomes (lepidochronology): present state and perspectives. Marine Ecology 1989, 10:221-230.
Roméo M, Gnassia-Barelli M, Juhel T, Meinesz A: Memorization of heavy metals by scales of the seagrass Posidonia oceanica, collected in the NW Mediterranean. Marine Ecology Progress Series 1995, 120:211-218.
Guidetti P, Fabiano M: The use of lepidochronology to assess the impact of terrigenous discharges on the primary leaf production of the Mediterranean seagrass Posidonia oceanica. Marine Pollution Bulletin 2000, 40:449-453.
Pergent G, Pergent-Martini C: Phenological study of Posidonia oceanica (Linneaus) Delile around the Mediterranean-sea. Annales de l'Institut Océanographique 1988, 64:79-100.
Warnau M, Ledent G, Temara A, Bouquegneau JM, Jangoux M, Dubois P: Heavy metals in Posidonia oceanica. and Paracentrotus lividus from seagrass beds of the north-western Mediterranean. Science of Total Environment 1995, 171:95-99.
Warnau M, Biondo R, Temara A, Bouquegneau JM, Jangoux M, Dubois P: Distribution of heavy metals in the echinoid Paracentrotus lividus from the Mediterranean Posidonia oceanica ecosystem: seasonal and geographical variations. Journal of Sea Research 1998, 39:267-280.
Sanchiz C, Garcia-Carrascosa AM, Pastor A: Heavy metal contents in soft-bottom marine macrophytes and sediments along the Mediterranean coast of Spain. Marine Ecology 2000, 21:1-16.
Campanella L, Conti ME, Cubadda F, Sucapane C: Trace metals in seagrass, algae and molluscs from an uncontamined area in the Mediterranean. Environmental Pollution 2001, 111:117-126.
Goffart A, Hecq JH, Legendre L: Changes in the development of the winter-spring phytoplankton bloom in the Bay of Calvi (NW Mediterranean) over the last two decades: a response to changing climate? Marine Ecology Progress Series 2002, 236:45-60.
Skliris N, Goffart A, Hecq JH, Djenidi S: Shelf-slope exchanges associated with a steep submarine canyon off Calvi (Corsica, NW Mediterranean Sea): modelling approach. Journal of Geophysical Research 2001, 106:19883-19201.
Giraud G: Essai de classement des herbiers de Posidonia oceanica (Linné) Delile. Botanica Marina 1977, 8:487-491.
Giraud G: Sur une méthode de mesure et de comptage des structures foliaires de Posidonia oceanica (Linnaeus) Delile. Bulletin Musée Histoire Naturelle Marseille France 1979, 39:33-39.
Pergent-Martini C, Rico-Raimondino V, Pergent G: Primary production of Posidonia oceanica meadows in the Mediterranean Basin. Marine Biology 1994, 120:9-15.
Gobert S, Laumont N, Bouquegneau JM: Posidonia oceanica meadow: a low nutrient high chlorophyll (LNHC) system. BioMed Central Ecology 2002, 2:9.
Wright DA, Welbourn P: Environnemental Toxicology Cambridge University Press; 2002.
Pasqualini V: Caractérisation des peuplements et types de fonds le long du littoral Corse (Méditerranée, France). In PhD thesis University of Corte, Eqel; 1997.
Pasqualini V, Pergent G, Pergent-Martini C: Utilisation de l'herbier à Posidonia oceanica comme indicateur biologique de la qualité du milieu littoral en Corse. Contrat agence de l'eau et Université de Corse 1999.
Claisse D, Alzieu C: Copper contamination as a result of antifouling paint regulation. Marine Pollution Bulletin 1993, 26:395-397.
Stephenson MD, Leonard GH: Evidence for the decline of silver and lead and the increase of copper from 1977 to 1990 in the coastal marine waters of california. Marine Pollution Bulletin 1994, 28:148-153.
Schlacher-Hoenlinger MA, Schlacher TA: Differential accumulation patterns of heavy metals among the dominant macrophytes of a Mediterranean seagrass meadow. Chemosphere 1998, 37:1511-1519.
Barwick M, Maher W: Biotransference and biomagnification of selenium, copper, cadmium, zinc, arsenic and lead in a temperate seagrass ecosystem from Lake Macquarie Estuary, NSW, Australia. Marine Environnemental Research 2003, 56:471-502.
Kaldy JE: Carbon, nitrogen, phosphorus and heavy metals budgets: How large is the eelgrass (Zostera marina L.) sink in a temperate estuary? Marine Pollution Bulletin in press.
Chatterjee A, Bhattacharya B, Das R: Temporal and organ-specific variability of selenium in marine organisms from the eastern coast of India. Advances in Environmental Research 2001, 5:167-174.
Baldwin S, Maher W, Kleber E, Krikowa F: Selenium in Marine Organisms of Seagrass Habitats (Posidonia australis) of Jervis Bay, Australia. Marine Pollution Bulletin 1996, 32:310-316.
Watanabe C: Modification of mercury toxicity by selenium: Practical importance? Tohoku Journal Of Experimental Medecine 2002, 196:71-77.
Baroli M, Cristini A, Cossu A, De Falco G, Gazale V, Pergent-Martini C, Pergent G: Concentrations of trace metals (Cd, Cu, Fe, Pb) in Posidonia oceanica seagrass of Lyscia Bay, Sardinia (Italy). In Structure and Processes in the Mediterranean Ecosystem Volume 14. Edited by: Faranda FM, Guglielmo L, Spezie G. Springer-Verlag; 2001:95-99.
Bouquegneau JM, Martoja M: Seasonal variation of the cadmium content of Murex trunculus in a non-cadmium polluted environment. Bulletin of Environmental Contamination and Toxicology 1987, 39:659-73.
Pergent-Martini C: Contribution à l'étude des stocks et des flux d'éléments dans l'écosystème à Posidonia oceanica. In Mémoire DESS University of Corte, Eqel; 1992.
Guilizzoni P: The role of heavy metals and toxic materials in the physiological ecology of submersed macrophytes. Aquatic Botany 1991, 41:87-109.
Pergent-Martini C: Impact d'un rejet d'eaux usées urbaines sur l'herbier à Posidonia oceanica, avant et après la mise en service d'une station d'épuration. In PhD thesis University of Corte, Eqel; 1994.
Lyngby JE, Brix H: The uptake of heavy metals in eelgrass Zostera marina and their effect on growth. Ecological Bulletins 1984, 36:81-89.
Malea P, Haritonidis S, Kevrekidis T: Seasonal and local concentrations in the seagrass Posidonia oceanica (L.) Delile in the Antikyra Gulf, Greece. The Science of the Total Environment 1994, 153:225-235.
Ridame C, Guiei C, Loye-Pilot MD: Trend in total atmospheric deposition fluxes of aluminium, iron and trace metals in the northwestern Mediterranean over the past decade (1985-1997). Journal of Geophysics Research 1999, 104:127-130.
Migon C, Nicolas E: Effects of Antipollution Policy on Anthropogenic Lead Transfers in the Ligurian Sea. Marine Pollution Bulletin 1998, 36:775-779.
Malea P, Haritonidis S: Uptake of Cu, Cd, Zn, and Pb in Posidonia oceanica (Linneaus) from Antikyra Gulf, Greece: preliminary note. Marine Environmental Research 1989, 28:495-498.
Capelli R, Drava G, Siccardi C, De Pellegrini R, Minganti V: Study of the distribution of trace elements in six species of marine organisms of the Ligurian Sea (North-Western Mediterranean) - Comparison with previous findings. Annali Di Chimica 2004, 94:533-546.
Jackson LJ, Kalff J, Rasmussen JB: Sediment pH and redox potential affect the bioavailability of Al, Cu, Fe, Mn, and Zn to rooted aquatic macrophytes. Canadian Journal of Fisheries and Aquatic Sciences 1993, 50:143-148.
Kljakovic-Gaspic Z, Antolic B, Zvonaric T, Baric A: Distribution of cadmium and lead in Posidonia oceanica (L.) delile from the middle Adriatic sea. Fresenius Environmental Bulletin 2004, 13:1210-1215.
Pergent-Martini C: Posidonia oceanica : a Biological Indicator of Past and Present Mercury Contamination in the Mediterranean Sea. Marine Environmental Reasearch 1998, 45:101-111.
Gagnon C: Efficacité malherbologique et devenir du cuivre de géotextiles imprégnés d'hydroxyde de cuivre. In M. Sc. Thesis University of Laval (Canada), Faculté des Sciences de l'Agriculture et de l'Alimentation, Département de Phytologie;; 1998.
Bethoux JP, Courau P, Nicolas E, Ruiz-Pino D: Trace metals pollution in the Mediterranean Sea. Oceanologica Acta 1990, 13:481-488.
Bethoux J, Morin P, Chaumery C, Connan O, Gentili B, Ruiz-Pino D: Nutrients in the Mediterranean Sea, mass balance and statistical analysis of concentrations with respect to environmental change. Marine Chemistry 1998, 63:155-169.
Delgado O, Ruiz J, Pérez M, Romero J, Ballesteros E: Effect of Fish farming on seagrass (Posidonia oceanica) in a Mediterranean bay: seagrass decline after organic loading cessation. Oceanologica Acta 1999, 22:109-117.
Gobert S, Kyramarios M, Lepoint G, Pergent-Martini G, Bouquegneau JM: Variations at different spatial scales of Posidonia oceanica (L.) Delile; Effects on the physico-chemical parameters on the sediment. Oceanologica Acta 2003, 26:199-207.
Soullard M, Bourge I, Fogel J, Lardinois D, Mathieu T, Veeschens C, Bay D, Dauby P, Bouquegneau JM: Evolution de la densité de l'herbier de Posidonies de la baie de Calvi (Corse). Vie Milieu 1994, 44:199-201.
Dauby P, Poulicek M: Methods for removing epiphytes from seagrasses: SEM observations on treated leaves. Aquatic Botany 1995, 52:217-228.
Boudouresque CF, Meinesz A: Découverte de l'herbier de Posidonie. Cahier du Parc national de Port-Cros 1982, 4:1-79.
