Article (Scientific journals)
Matrix Metalloproteinases and TIMP-1 production by peripheral blood granulocytes from COPD patients and asthmatics
Cataldo, Didier; Munaut, Carine; Noël, Agnès et al.
2001In Allergy, 56 (2), p. 145-51
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Abstract :
[en] Both asthmatic and COPD patients were found to have increased amounts of granulocytes and matrix metalloproteinase-9 (MMP-9) in their sputum. The present study was conducted to investigate whether the elevated amounts of MMP-9 and TIMP-1 found in such patients' airways may be linked to an enhanced secretion by granulocytes. Blood granulocytes from asthmatics (n = 10), COPD patients (n = 11), and healthy controls (n = 11) were isolated and cultured under basal conditions or after stimulation by phorbol 12-myristate 13-acetate (PMA) or N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP). MMP-9 activity was detected by zymography while MMP-8 and TIMP-1 levels were measured by ELISA. In zymography, pro- and activated forms of MMP-9 were present in each group (healthy subjects, asthmatics, and COPD patients). Spontaneous release was not different between the three groups. Stimulation by fMLP and PMA increased to a similar extent the release of MMP-9 by granulocytes in all the three groups. TIMP-1 levels were also increased after stimulation by PMA and fMLP only in healthy subjects and COPD patients. MMP-8 levels were barely detectable. We conclude that circulating granulocytes from COPD patients and asthmatics do not display an abnormal secretion of MMP-9, and that granulocytes from asthmatics have an impaired ability to release TIMP-1 upon stimulation.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Cataldo, Didier  ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement - Département des sciences biomédicales et précliniques
Munaut, Carine  ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Noël, Agnès ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Frankenne, Francis
Bartsch, Pierre ;  Université de Liège - ULiège > Pneumologie-Allergologie
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Louis, Renaud ;  Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie
Language :
English
Title :
Matrix Metalloproteinases and TIMP-1 production by peripheral blood granulocytes from COPD patients and asthmatics
Publication date :
February 2001
Journal title :
Allergy
ISSN :
0105-4538
eISSN :
1398-9995
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
56
Issue :
2
Pages :
145-51
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 May 2009

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