Reference : Nucleolar changes and fibrillarin redistribution following apatone treatment of human...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Nucleolar changes and fibrillarin redistribution following apatone treatment of human bladder carcinoma cells.
Jamison, James M [> > > >]
Gilloteaux, Jacques [> > > >]
Perlaky, Laszlo [> > > >]
Thiry, Marc mailto [Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire]
Smetana, Karel [> > > >]
Neal, Deborah [> > > >]
McGuire, Karen [> > > >]
Summers, Jack L [> > > >]
Journal of Histochemistry and Cytochemistry
Histochemical Society
Yes (verified by ORBi)
New York
[en] Antineoplastic Combined Chemotherapy Protocols/pharmacology ; Ascorbic Acid/pharmacology ; Carcinoma/chemistry/ultrastructure ; Cell Line, Tumor ; Cell Nucleolus/chemistry/drug effects/ultrastructure ; Chromosomal Proteins, Non-Histone/analysis ; Humans ; Urinary Bladder Neoplasms/chemistry/ultrastructure ; Vitamin K/pharmacology ; Vitamin K 3/pharmacology
[en] Ascorbate and menadione (Apatone) in a ratio of 100:1 kills tumor cells by autoschizis. In this study, vitamin-induced changes in nucleolar structure were evaluated as markers of autoschizis. Human bladder carcinoma (T24) cells were overlain with vitamins or with culture medium. Supernatants were removed at 1-hr intervals from 1 to 4 hr, and the cells were washed with PBS and prepared for assay. Apatone produced marked alterations in nucleolar structure including redistribution of nucleolar components, formation of ring-shaped nucleoli, condensation and increase of the proportion of perinucleolar chromatin, and the enlargement of nucleolar fibrillar centers. Immunogold labeling of the nucleolar rRNA revealed a granular localization in treated and sham-treated cells, and immunogold labeling of the rDNA revealed a shift from the fibrillar centers to the condensed perinucleolar chromatin. Fibrillarin staining shifted from the fibrillar centers and adjacent regions to a more homogeneous staining of the entire nucleolus and was consistent with the percentage of autoschizic cells detected by flow cytometry. Because autoschizis entails sequential reactivation of DNase I and DNase II, and because the fibrillarin redistribution following DNase I and Apatone treatment is identical, it appears that the nucleolar and fibrillarin changes are markers of autoschizis.

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