Reference : Detection of disease resistance and susceptibility alleles in pigs using oligonucleot...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Detection of disease resistance and susceptibility alleles in pigs using oligonucleotide microarray hybridization
Pastoret, Soumya [ > > ]
Ameels, Hélène [ > > ]
Bossiroy, Frédérique [ > > ]
Decreux, Annabelle [ > > ]
De Longueville, Françoise [ > > ]
Thomas, Anne [ > > ]
Remacle, José [ > > ]
Desmecht, Daniel mailto [Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
Journal of Veterinary Diagnostic Investigation
American Association of Veterinary Laboratory Diagnosticians (Aavld)
Yes (verified by ORBi)
[en] microarray ; marker-assisted selection ; pig
[en] A multiplex DNA microarray chip aimed at the identification of allelic polymorphisms was developed for simultaneous detection of swine disease resistance genes underlying malignant hyperthermia (RYR ), postweaning diarrhea, edema disease (FUT1), neonatal diarrhea (MUC4), and influenza (MX1). The on-chip detection was performed with fragmented polymerase chain reaction (PCR)-amplified products. Particular emphasis was placed on the reduction of the number of PCR reactions required. The targets were biotin labeled during the PCR reaction, and the arrays were detected using a colorimetric methodology. Target recognition was provided by specific capture probes designed for each susceptible or resistant allelic variant. Sequencing was chosen as the golden standard to assess chip’s accuracy. All genotypes retrieved from the microarray (476) fitted with sequencing data in spite of the fact that each pig was heterozygote for at least 1 target gene.
Researchers ; Professionals

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