Article (Scientific journals)
Detection of disease resistance and susceptibility alleles in pigs using oligonucleotide microarray hybridization
Pastoret, Soumya; Ameels, Hélène; Bossiroy, Frédérique et al.
2012In Journal of Veterinary Diagnostic Investigation, 24 (3), p. 479-488
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Keywords :
microarray; marker-assisted selection; pig
Abstract :
[en] A multiplex DNA microarray chip aimed at the identification of allelic polymorphisms was developed for simultaneous detection of swine disease resistance genes underlying malignant hyperthermia (RYR ), postweaning diarrhea, edema disease (FUT1), neonatal diarrhea (MUC4), and influenza (MX1). The on-chip detection was performed with fragmented polymerase chain reaction (PCR)-amplified products. Particular emphasis was placed on the reduction of the number of PCR reactions required. The targets were biotin labeled during the PCR reaction, and the arrays were detected using a colorimetric methodology. Target recognition was provided by specific capture probes designed for each susceptible or resistant allelic variant. Sequencing was chosen as the golden standard to assess chip’s accuracy. All genotypes retrieved from the microarray (476) fitted with sequencing data in spite of the fact that each pig was heterozygote for at least 1 target gene.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Pastoret, Soumya
Ameels, Hélène
Bossiroy, Frédérique
Decreux, Annabelle
De Longueville, Françoise
Thomas, Anne
Remacle, José
Desmecht, Daniel ;  Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Language :
English
Title :
Detection of disease resistance and susceptibility alleles in pigs using oligonucleotide microarray hybridization
Publication date :
May 2012
Journal title :
Journal of Veterinary Diagnostic Investigation
ISSN :
1040-6387
Publisher :
American Association of Veterinary Laboratory Diagnosticians (Aavld), Columbia, United States - Missouri
Volume :
24
Issue :
3
Pages :
479-488
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 March 2012

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