[en] BACKGROUND: Myeloperoxidase (MPO) is a member of the peroxidase-cyclooxygenase superfamily, which is secreted from stimulated leucocytes at inflammatory sites. It is well known that MPO catalyses oxidation reactions via the release of reactive halogenating and nitrating species and thus induces tissue damage. Several studies have already implicated MPO in the development of neoplasia. Chronic or recurrent prostatic inflammation has long been recognized as having the potential to initiate and promote the development of prostate cancer. The objective was to investigate whether MPO is present in the prostate. METHODS: Human prostate material was obtained from biopsies, transurethral resections of the prostate (TURP), prostatic adenomectomies, and retropubic radical prostatectomies. Twenty-nine slides of normal prostate tissue, benign prostatic hyperplasia (BPH), and prostate cancer were reviewed by a pathologist. Immunohistochemical analysis using MPO-specific human antibody was performed to detect MPO in the prostate tissue. RESULTS: Immunocytohistochemistry showed cellular colocalization of MPO in the secretory epithelial cells of the prostate with staining varying from light to strong intensity. Staining in the glandular apical snouts was often reinforced although staining of basal as well as of luminal glandular cells was also present. CONCLUSIONS: We identified, for the first time, the presence of MPO at the surface of prostatic epithelial cells. In view of the pro-oxidant properties of this enzyme, further research is needed to define whether MPO contributes to the development of prostatic lesions. Prostate 72:507-513, 2012. (c) 2011 Wiley Periodicals, Inc.
Research Center/Unit :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Roumeguere, Thierry; Université Libre de Bruxelles - ULB > CHU Charleroi > Laboratory of Experimental Medicine
Delree, Paul; Institute of Pathologyand Genetics (IPG),Gosselies, Belgium
Van Antwerpen, Pierre; Université Libre de Bruxelles - ULB > Faculty of Pharmacy > Laboratory of Pharmaceutical Chemistry and Analytical Platform
Rorive, S.
Vanhamme, L.
de Ryhove, L de la Kethulle
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Wespes, E.
Vanhaerverbeek, M.
Boudjeltia, K Zouaoui; Université Libre de Bruxelles - ULB > CHU Charleroi > Laboratory of Experimental Medicine
Language :
English
Title :
Intriguing location of myeloperoxidase in the prostate: A preliminary immunohistochemical study.
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