[en] Eukaryotic phosphomannomutases (PMMs) catalyze the interconversion of mannose 6-phosphate to mannose 1-phosphate and are essential to the biosynthesis of GDP-mannose. As such, plant PMMs are involved in ascorbic acid (AsA) biosynthesis and N-glycosylation. We report on the conditional phenotype of the temperature-sensitive Arabidopsis thaliana pmm-12 mutant. Mutant seedlings were phenotypically similar to wild type seedlings when grown at 16-18 degrees C but died within several days after transfer to 28 degrees C. This phenotype was observed throughout both vegetative and reproductive development. Protein extracts derived from pmm-12 plants had lower PMM protein and enzyme activity levels. In vitro biochemical analysis of recombinant proteins showed that the mutant PMM protein was compromised in its catalytic efficiency (K cat/K m). Despite significantly decreased AsA levels in pmm-12 plants, AsA deficiency could not account for the observed phenotype. Since, at restrictive temperature, total glycoprotein patterns were altered and glycosylation of protein-disulfide isomerase was perturbed, we propose that a deficiency in protein glycosylation is responsible for the observed cell death phenotype.
Hoeberichts, Frank A; Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology
Vaeck, Elke; Flanders Institute for Biotechnology - VIB > Departement of Plant Systems Biology
Kiddle, Guy; Rothamsted Research > Crop Performance and Improvement Division
Coppens, Emmy; Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology
van de Cotte, Brigitte; Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology
Adamantidis, Antoine ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
Ormenese, Sandra ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie végétale > Physiologie végétale
Foyer, Christine H; Rothamsted Research > Crop Performance and Improvement Division
Zabeau, Marc; Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology
Inze, Dirk; Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology
Périlleux, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie végétale
Van Breusegem, Frank; Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology
Vuylsteke, Marnik; Flanders Institute for Biotechnology (VIB) > Department of Plant Systems Biology
A Temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death.
Publication date :
2008
Journal title :
Journal of Biological Chemistry
ISSN :
0021-9258
eISSN :
1083-351X
Publisher :
American Society for Biochemistry and Molecular Biology, Baltimore, United States - Maryland
Volume :
283
Issue :
9
Pages :
5708-18
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique Research Fund of the Ghent University Research Foundation-Flanders
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Seifert, G. J. (2004) Curr. Opin. Plant Biol. 7, 277-284
Wheeler, G. L., Jones, M. A., and Smirnoff, N. (1998) Nature 393, 365-369
Qian, W., Yu, C., Qin, H., Liu, X., Zhang, A., Johansen, I. E., and Wang, D. (2007) Plant J. 49, 399-413
Lerouge, P., Cabanes-Macheteau, M., Rayon, C., Fischette-Lainé, A.-C., Gomord, V., and Faye, L. (1998) Plant Mol. Biol. 38, 31-48
Spiro, R. G. (2002) Glycobiology 12, 43R-56R
Bernstein, M., Hoffmann, W., Ammerer, G., and Schekman, R. (1985) J. Cell Biol. 101, 2374-2382
Kepes, F., and Schekman, R. (1988) J. Biol. Chem. 263, 9155-9161
Garami, A., Mehlert, A., and Ilg, T. (2001) Mol. Cell. Biol. 21, 8168-8183
Thiel, C., Lübke, T., Matthijs, G., von Figura, K., and Körner, C. (2006) Mol. Cell. Biol. 26, 5615-5620
Van Schaftingen, E., and Jaeken, J. (1995) FEBS Lett. 377, 318-320
Conklin, P. L., Norris, S. R., Wheeler, G. L., Williams, E. H., Smirnoff, N., and Last, R. L. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 4198-4203
Conklin, P. L., Pallanca, J. E., Last, R. L., and Smirnoff, N. (1997) Plant Physiol. 115, 1277-1285
Karimi, M., Inzé, D., and Depicker, A. (2002) Trends Plant Sci. 7, 193-195
Clough, S. J., and Bent, A. F. (1998) Plant J. 16, 735-743
Peters, J. L., Cnops, G., Neyt, P., Zethof, J., Cornelis, K., Van Lijsebettens, M., and Gerats, T. (2004) Theor. Appl. Genet. 108, 321-327
Peters, J. L., Constandt, H., Neyt, P., Cnops, G., Zethof, J., Zabeau, M., and Gerats, T. (2001) Plant Physiol. 127, 1579-1589
Vuylsteke, M., Peleman, J. D., and van Eijk, M. J. T. (2007) Nat. Protocols 2, 1387-1398
Jander, G., Norris, S. R., Rounsley, S. D., Bush, D. F., Levin, I. M., and Last, R. L. (2002) Plant Physiol. 129, 440-450
Rozen, S., and Skaletsky, H. (2000) Methods Mol. Biol. 132, 365-386
Bradford, M. M. (1976) Anal. Biochem. 72, 248-254
Hancock, R. D., McRae, D., Haupt, S., and Viola, R. (2003) BMC Plant Biol. 3, 7.1-7.13
Vernon, L. P. (1960) Anal. Chem. 32, 1144-1150
Foyer, C., Rowell, J., and Walker, D. (1983) Planta 157, 239-244
Lukowitz, W., Nickle, T. C., Meinke, D. W., Last, R. L., Conklin, P. L., and Somerville, C. R. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 2262-2267
Vaeck, E. (2006) Ph.D. thesis, Ghent University, Gent, Belgium
Bell, C. J., and Ecker, J. R. (1994) Genomics 19, 137-144
Koonin, E. V., and Tatusov, R. L. (1994) J. Mol. Biol. 244, 125-132
Collet, J.-F., Stroobant, V., Pirard, M., Delpierre, G., and Van Schaftingen, E. (1998) J. Biol. Chem. 273, 14107-14112
Rosso, M. G., Li, Y., Strizhov, N., Reiss, B., Dekker, K., and Weisshaar, B. (2003) Plant Mol. Biol. 53, 247-259
Faye, L., and Chrispeels, M. J. (1985) Anal. Biochem. 149, 218-224
Lerouxel, O., Mouille, G., Andème-Onzighi, C., Bruyant, M.-P., Séveno, M., Loutelier-Bourhis, C., Driouich, A., Höfte, H., and Lerouge, P. (2005) Plant J. 42, 455-468
Shorrosh, B. S., Subramaniam, J., Schubert, K. R., and Dixon, R. A. (1993) Plant Physiol. 103, 719-726
Shimoni, Y., Zhu, X.-z., Levanony, H., Segal, G., and Galili, G. (1995) Plant Physiol. 108, 327-335
Kinoshita, T., and Inoue, N. (2000) Curr. Opin. Chem. Biol. 4, 632-638
Gillmor, C. S., Lukowitz, W., Brininstool, G., Sedbrook, J. C., Hamann, T., Poindexter, P., and Somerville, C. (2005) Plant Cell 17, 1128-1140
Morais, M. C., Zhang, W., Baker, A. S., Zhang, G., Dunaway-Mariano, D., and Allen, K. N. (2000) Biochemistry 39, 10385-10396
Silvaggi, N. R., Zhang, C., Lu, Z., Dai, J., Dunaway-Mariano, D., and Allen, K. N. (2006) J. Biol. Chem. 281, 14918-14926
Jones, D. T. (1999) J. Mol. Biol. 292, 195-202
McGuffin, L. J., and Jones, D. T. (2003) Bioinformatics 19, 874-881
Hashimoto, H., Sakakibara, A., Yamasaki, M., and Yoda, K. (1997) J. Biol. Chem. 272, 16308-16314
Kukuruzinska, M. A., and Lennon-Hopkins, K. (1999) Biochim. Biophys. Acta 1426, 359-372
Nakashima, T., Sekiguchi, T., Kuraoka, A., Fukushima, K., Shibata, Y., Komiyama, S., and Nishimoto, T. (1993) Mol. Cell. Biol. 13, 6367-6374
Silberstein, S., Collins, P. G., Kelleher, D. J., and Gilmore, R. (1995) J. Cell Biol. 131, 371-383
Boisson, M., Gomord, V., Audran, C., Berger, N., Dubreucq, B., Granier, F., Lerouge, P., Faye, L., Caboche, M., and Lepiniec, L. (2001) EMBO J. 20, 1010-1019
Gillmor, C. S., Poindexter, P., Lorieau, J., Palcic, M. M., and Somerville, C. (2002) J. Cell Biol. 156, 1003-1013
Koiwa, H., Li, F., McCully, M. G., Mendoza, I., Koizumi, N., Manabe, Y., Nakagawa, Y., Zhu, J., Rus, A., Pardo, J. M., Bressan, R. A., and Hasegawa, P. M. (2003) Plant Cell 15, 2273-2284
Conklin, P. L., Williams, E. H., and Last, R. L. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 9970-9974
Wolucka, B. A., and Van Montagu, M. (2007) Phytochemistry 68, 2602-2613
Dowdle, J., Ishikawa, T., Gatzek, S., Rolinski, S., and Smirnoff, N. (2007) Plant J. 52, 673-689
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