A comparison of bovine growth hormone expression directed by bGH genomic or intronless DNA in transiently transfected eukaryotic cells.

Pasleau, Françoise; Leung, F. C.; Kopchick, J. J.

doi:10.1016/0378-1119(87)90175-2

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A comparison of bovine growth hormone expression directed by bGH genomic or intronless DNA in transiently transfected eukaryotic cells.

Pasleau, Françoise; Leung, F. C.; Kopchick, J. J.

1987 • In Gene, 57, p. 47-52

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https://hdl.handle.net/2268/111887

DOI
10.1016/0378-1119(87)90175-2

PubMed
2828181

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Abstract :

[en] Two recombinant DNA plasmids were constructed with identical transcriptional and translational regulatory elements controlling expression of the bovine growth hormone (bGH) gene or the bGH gene lacking introns. Transient expression of these plasmids in cultured eukaryotic cells, monitored by assaying secretion of bGH into the culture medium, was employed to examine the relative importance of introns in the expression of this gene. The bGH gene lacking introns is expressed more efficiently than the bGH gene in avian and mammalian cells.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Pasleau, Françoise ; Université de Liège - ULiège > CARE "Le Réseau des bibliothèques" > Bibliothèque des Sciences de la vie

Leung, F. C.

Kopchick, J. J.

Language :

English

Title :

A comparison of bovine growth hormone expression directed by bGH genomic or intronless DNA in transiently transfected eukaryotic cells.

Publication date :

1987

Journal title :

Gene

ISSN :

0378-1119

eISSN :

1879-0038

Publisher :

Elsevier, Amsterdam, Netherlands

Volume :

Pages :

47-52

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 15 February 2012

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Bibliography

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