Reference : Processing samples of benthic marine diatoms from Mediterranean oligotrophic areas
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Life sciences : Aquatic sciences & oceanology
Life sciences : Environmental sciences & ecology
Processing samples of benthic marine diatoms from Mediterranean oligotrophic areas
Vermeulen, Simon mailto [Université de Liège - ULiège > > > Doct. sc. (océanogr. - Bologne)]
Lepoint, Gilles mailto [Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanologie >]
Gobert, Sylvie mailto [Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanologie >]
Journal of Applied Phycology
Yes (verified by ORBi)
[en] Bacillariophyta ; Methods ; Cell losses ; Cleaning techniques ; Silicification
[en] The processing of benthic diatoms is tedious and involves several potentially damaging steps for cells. Although the preservation of siliceous frustules is of paramount importance in the implementation of biotic indices, only few studies quantified treatment-induced cell losses. We assumed that commonly used treatments may lead to mechanical (centrifugation, sedimentation, boiling, sonication and mounting in Naphrax) and chemical (cold H2O2 digestion) damages on diatoms. We analysed the potential adverse effects of these treatments and the cleaning efficiency of H2O2 and incineration in order to find out the most suitable technique to process lightly silicified Mediterranean populations. Results showed that successive resuspensions of material after each concentration treatment (sedimentation and centrifugation) and low speed centrifugation did not alter the physical integrity of frustules. In contrast, boiling and sonication exhibited adverse effects especially on the preservation of large frustules and Naphrax mounting proved to be the most damaging step whatever the size of diatoms. For cleaning treatments, incineration provided the most satisfactory results and acted on a non-selective way as opposed to hydrogen peroxide which led to either a large number of non-cleaned frustules or dissolved valves. Our recommendations for processing samples of lightly silicified Mediterranean benthic diatoms include the use of low speed centrifugations, dehydration at room temperature, incineration and dry mounting.
Centre Interfacultaire de Recherches en Océanologie - MARE
Belgian National Fund for Scientific Research ; French Belgian community
ARC - RACE 05/10-333
Researchers ; Professionals ; Students
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