Article (Scientific journals)
The varicella-zoster virus immediate-early 63 protein affects chromatin-controlled gene transcription in a cell-type dependent manner.
Habran, Lionel; El Mjiyad, Nadia; Di Valentin, Emmanuel et al.
2007In BMC Molecular Biology, 8, p. 99
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Keywords :
Varicella zoster virus; NF-KB
Abstract :
[en] Varicella Zoster Virus Immediate Early 63 protein (IE63) has been shown to be essential for VZV replication, and critical for latency establishment. The activity of the protein as a transcriptional regulator is not fully clear yet. Using transient transfection assays, IE63 has been shown to repress viral and cellular promoters containing typical TATA boxes by interacting with general transcription factors. In this paper, IE63 regulation properties on endogenous gene expression were evaluated using an oligonucleotide-based micro-array approach. We found that IE63 modulates the transcription of only a few genes in HeLa cells including genes implicated in transcription or immunity. Furthermore, we showed that this effect is mediated by a modification of RNA POL II binding on the promoters tested and that IE63 phosphorylation was essential for these effects. In MeWo cells, the number of genes whose transcription was modified by IE63 was somewhat higher, including genes implicated in signal transduction, transcription, immunity, and heat-shock signalling. While IE63 did not modify the basal expression of several NF-κB dependent genes such as IL-8, ICAM-1, and IκBα, it modulates transcription of these genes upon TNFα induction. This effect was obviously correlated with the amount of p65 binding to the promoter of these genes and with histone H3 acetylation and HDAC-3 removal. Conclusion While IE63 only affected transcription of a small number of cellular genes, it interfered with the TNF-inducibility of several NF-κB dependent genes by the accelerated resynthesis of the inhibitor IκBα.
Research center :
GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Habran, Lionel ;  Université de Liège - ULiège > Sciences de la Vie > GIGA Research Virologie -Immunologie
El Mjiyad, Nadia ;  Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie GIGA-Research
Di Valentin, Emmanuel  ;  Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie GIGA-Research
Sadzot-Delvaux, Catherine ;  Université de Liège - ULiège > Département des sciences de la vie > Virologie et immunologie - GIGA-Research
Bontems, Sébastien ;  Université de Liège - ULiège > Sciences de la Vie > GIGA-Research Virologie - Immunologie
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie - GIGA-Research
Language :
English
Title :
The varicella-zoster virus immediate-early 63 protein affects chromatin-controlled gene transcription in a cell-type dependent manner.
Publication date :
October 2007
Journal title :
BMC Molecular Biology
eISSN :
1471-2199
Publisher :
BioMed Central
Volume :
8
Pages :
99
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 25 November 2008

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