The structure at 2 A resolution of Phycocyanin from Gracilaria chilensis and the energy transfer network in a PC-PC complex.

Contreras-Martel, Carlos; Matamala, Adelio; Bruna, Carola; Poo-Caamano, German; Almonacid, Daniel; Figueroa, Maximiliano; Martinez-Oyanedel, Jose; Bunster, Marta

doi:10.1016/j.bpc.2006.09.014

Article (Scientific journals)

The structure at 2 A resolution of Phycocyanin from Gracilaria chilensis and the energy transfer network in a PC-PC complex.

Contreras-Martel, Carlos; Matamala, Adelio; Bruna, Carola et al.

2007 • In Biophysical Chemistry, 125 (2-3), p. 388-96

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/111234

DOI
10.1016/j.bpc.2006.09.014

PubMed
17118524

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Keywords :

Crystallography, X-Ray; Energy Transfer; Gracilaria/chemistry; Macromolecular Substances/chemistry; Molecular Structure; Phycocyanin/chemistry

Abstract :

[en] Phycocyanin is a phycobiliprotein involved in light harvesting and conduction of light to the reaction centers in cyanobacteria and red algae. The structure of C-phycocyanin from Gracilaria chilensis was solved by X-ray crystallography at 2.0 A resolution in space group P2(1). An interaction model between two PC heterohexamers was built, followed by molecular dynamic refinement. The best model showed an inter-hexamer rotation of 23 degrees . The coordinates of a PC heterohexamer (alphabeta)(6) and of the PC-PC complex were used to perform energy transfer calculations between chromophores pairs using the fluorescence resonance energy transfer approach (FRET). Two main intra PC ((I)beta(3)(82)-->(I)alpha(1)(84)-->(I)alpha(5)(84)-->(I)beta(6)(82) and (I)beta(3)(153)-->(I)beta(5)(153)) and two main inter PC ((I)beta(6)(82)-->(II)beta(3)(82) and (I)beta(5)(153)-->(II)beta(3)(153)) pathways were proposed based on the values of the energy transfer constants calculated for all the chromophore pairs in the hexamer and in the complex.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Contreras-Martel, Carlos

Matamala, Adelio

Bruna, Carola

Poo-Caamano, German

Almonacid, Daniel

Figueroa, Maximiliano ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Martinez-Oyanedel, Jose

Bunster, Marta

Language :

English

Title :

The structure at 2 A resolution of Phycocyanin from Gracilaria chilensis and the energy transfer network in a PC-PC complex.

Publication date :

2007

Journal title :

Biophysical Chemistry

ISSN :

0301-4622

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

125

Issue :

2-3

Pages :

388-96

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 February 2012

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