[en] This paper describes two new immunoassays for a peptide of the triple helix of type II collagen (Coll 2-1) and its nitrated form (Coll 2-1 NO2). In healthy subjects aged between 20 and 65 years old, Coll 2-1 and Coll 2-1 NO2 levels in serum were in means 125.13+/-3.71 and 0.16+/-0.08 nmol/l, respectively. These levels did not significantly vary with age. However, up to 45 years of age, Coll 2-1 NO2 levels in women were significantly higher than in men. In patients with knee osteoarthritis (OA), Coll 2-1 in serum was found to be elevated compared to healthy controls (267.45+/-26.42 nmol/l vs 126.78+/-6.61 nmol/l). Further, we have demonstrated that an increase of the urinary levels of Coll 2-1 or Coll 2-1 NO2 over 1 year was predictive of joint space narrowing progression in OA patients. In conclusion, these preliminary results indicate that Coll 2-1 could be a predictive marker of knee OA progression.
Disciplines :
Laboratory medicine & medical technology Hematology Biochemistry, biophysics & molecular biology
Author, co-author :
Henrotin, Yves ; Université de Liège - ULiège > Unité de recherche sur l'os et le cartillage (U.R.O.C.)
Deberg, Michelle ; Université de Liège - ULiège > Unité de recherche sur l'os et le cartillage (U.R.O.C.)
Dubuc, J. E.
Quettier, E.
Christgau, S.
Reginster, Jean-Yves ; Université de Liège - ULiège > Département des sciences de la santé publique > Epidémiologie et santé publique
Language :
English
Title :
Type II collagen peptides for measuring cartilage degradation
Billinghurst, R.C., Dahlberg, L., Ionescu, M., Reiner, A., Bourne, R., Rorabeck, C., Mitchell, P., Poole, R., Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage (1997) J. Clin. Invest., 99, pp. 1534-1545
Deberg, M., Christgau, S., Cloos, P., Henriksen, D.B., Sanchez, C., Labasse, A., Reginster, J.Y., Henrotin, Y., Development of new immunoassays for the quantification of inflammatory related cartilage degradation (2002) Arthritis Rheum., 46, pp. S496. , P1319
Garnero, P., Piperno, M., Gineyts, E., Christgau, S., Delmas, P.D., Vignon, E., Cross sectional evaluation of biochemical markers of bone, cartilage and synovial tissue metabolism in patients with knee osteoarthritis: Relations with disease activity and joint damage (2001) Ann. Rheum. Dis., 60, pp. 619-626
Garnero, P., Ayral, X., Rousseau, J.C., Christgau, S., Sandell, L., Dougados, M., Delmas, P.D., Uncoupling of type II collagen synthesis and degradation predicts progression of joint damage in patients with knee osteoarthritis (2002) Arthritis Rheum., 46, pp. 2613-2624
Garnero, P., Gineyts, E., Christgau, S., Finck, B., Delmas, P., Association of baseline levels of urinary glucosyl-galactosyl- pyridinoline and type II collagen C-telopeptide with progression of joint destruction in patients with early rheumatoid arthritis (2002) Arthritis Rheum., 46, pp. 21-30
Henrotin, Y., Deberg, M., Christgau, S., Henriksen, D.B., Seidel, L., Reginster, J.Y., Type II collagen derived fragment (Coll 2-1) is a new marker predictive of osteoarthritis progression (2002) Osteoporosis Int., 13, pp. S17. , 021
Henrotin, Y., Deby-Dupont, G., Deby, C., De Bruyn, M., Lamy, M., Franchimont, P., Production of active oxygen species by isolated human chondrocytes (1993) Br. J. Rheumatol., 32, pp. 562-567
Henrotin, Y.E., Zheng, S.X., Deby, G.P., Labasse, A.H., Crielaard, J.M.R., Reginster, J.Y., Nitric oxide downregulates interleukin 1β (IL-1β) stimulated IL-6, IL-8, and prostaglandin E2 production by human chondrocytes (1998) J. Rheumatol., 25, pp. 1595-1601
Hollander, A.P., Heathfield, T.F., Webber, C., Iwata, Y., Bourne, R., Rorabeck, C., Poole, A.R., Increased damage to type II collagen in osteoarthritic articular cartilage detected by a new immunoassay (1994) J. Clin. Invest., 93, pp. 1722-1732
Nelson, F., Dahlberg, L., Laverty, S., Reiner, A., Pidoux, I., Ionescu, M., Fraser, G.L., Poole, R., Evidence for altered synthesis of type II collagen in patients with osteoarthritis (1998) J. Clin. Invest., 102, pp. 2115-2125
Otterness, I.G., Downs, J.T., Lane, C., Bliven, M.L., Stukenbrok, H., Scampoli, D.N., Milici, A.J., Mèzes, P.S., Detection of collagenase-induced damage of collagen by 9A4, a monoclonal C-terminal neoepitope antibody (1999) Matrix Biology, 18, pp. 331-341
Reginster, J.Y., Deroisy, R., Rovati, L.C., Lee, R.L., Lejeune, E., Bruyere, O., Giacovelli, G., Gossett, C., Long-term effects of glucosamine sulphate on osteoarthritis progression: A randomised, placebo-controlled trial (2001) The Lancet, 357, pp. 251-256
Thonar, E.J.M.A., Masuda, K., Manicourt, D.H., Kuettner, K.E., Structure and function of normal adult articular cartilage (1999) Osteoarthritis: Clinical and Experimental Aspects, pp. 1-19. , J. Y. Reginster, J.P. Pelletier, J. Martel-Pelletier and Y. Henrotin, eds, Springer, Berlin
Van Der Vliet, A., Smith, D., O'Neill, C.A., Kaur, H., Darley-Usmar, V., Cross, C.E., Halliwell, B., Interactions of peroxynitrite with human plasma and its constituents: Oxidative damage and antioxidant depletion (1994) Biochem. J., 303, pp. 295-301
