Protein-protein interactions and networks: forward and reverse edgetics.

[en] Phenotypic variations of an organism may arise from alterations of cellular networks, ranging from the complete loss of a gene product to the specific perturbation of a single molecular interaction. In interactome networks that are modeled as nodes (macromolecules) connected by edges (interactions), these alterations can be thought of as node removal and edge-specific or "edgetic" perturbations, respectively. Here we present two complementary strategies, forward and reverse edgetics, to investigate the phenotypic outcomes of edgetic perturbations of binary protein-protein interaction networks. Both approaches are based on the yeast two-hybrid system (Y2H). The first allows the determination of the interaction profile of proteins encoded by alleles with known phenotypes to identify edgetic alleles. The second is used to directly isolate edgetic alleles for subsequent in vivo characterization.

Disciplines :

Genetics & genetic processes

Author, co-author :

Charloteaux, Benoît ; Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale

Zhong, Quan

Dreze, Matija

Cusick, Michael E

Hill, David E

Vidal, Marc

Language :

English

Title :

Protein-protein interactions and networks: forward and reverse edgetics.

Publication date :

2011

Main work title :

Yeast Systems Biology, Methods in Molecular Biology 759

Editor :

Castrillo, J. I.

Oliver, S. G.

Collection name :

Methods in Molecular Biology 759

Pages :

197-213

Available on ORBi :

since 29 January 2012

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Bibliography

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