Regulation of CXCL8/IL-8 expression by Zonula Occludens-1 in human breast cancer cells.

[en] Accumulating data now suggest that ZO-1, once delocalized from tight junctions, could be implicated in the regulation of tumor promoting genes. Because of their major implication in different steps of tumor progression, we investigated here the influence of ZO-1 on chemokines expression in breast cancer cells. Using GeneArray analysis to compare chemokine mRNA expression in breast tumor cells transfected with a siRNA against ZO-1, we identified CXCL-8/IL-8 as a major potential target of ZO-1 signaling, being strongly downregulated following ZO-1 siRNA transfection. Examining further the relationship between ZO-1 and IL-8, we first demonstrated that CXCL8/IL-8 expression correlates with a relocalization of ZO-1 in several breast cancer cell lines. Moreover, CXCL8/IL-8 is downregulated in invasive BT549 cells transfected with 3 different ZO-1 siRNA and overexpressed in non-invasive BT20 and SKBR3 cells transfected with vectors expressing ZO-1. We also provide evidence for an activation of the CXCL8/IL-8 promoter by ZO-1. Finally, we demonstrate that the regulation of CXCL8/IL-8 by ZO-1 is independent of the beta-catenin pathway. Our results thus clearly demonstrate an implication of ZO-1 in CXCL8/IL-8 regulation. Because of the major implications of CXCL8/IL-8 in tumor invasion, such a regulation could play an important role in breast cancer progression.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology
Biochemistry, biophysics & molecular biology

Author, co-author :

Brysse, Anne ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

Mestdagt, Mélanie ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

Polette, Myriam

Luczka, E.

Hunziker, W.

Noël, Agnès ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme

Birembaut, P.

Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique

Gilles, Christine ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

Language :

English

Title :

Regulation of CXCL8/IL-8 expression by Zonula Occludens-1 in human breast cancer cells.

Publication date :

2012

Journal title :

Molecular Cancer Research

ISSN :

1541-7786

eISSN :

1557-3125

Publisher :

American Association for Cancer Research, Inc. (AACR)

Volume :

Issue :

Pages :

121-32

Peer reviewed :

Peer Reviewed verified by ORBi

European Projects :

FP7 - 201279 - MICROENVIMET - Understanding and fighting metastasis by modulating the tumour microenvironment through interference with the protease network.

Funders :

CE - Commission Européenne

Available on ORBi :

since 25 January 2012

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