[en] Olfactory ensheathing cells (OECs) together with olfactory nerve fibroblasts (ONFs) and neonatal astrocytes are potent stimulators of neurite growth in adulthood and during development, respectively. Since it is known that alignment of glial cells is important for the correct outgrowth of axon tracts, it was hypothesized that the alignment of glial cells stimulates directional and enhanced neurite outgrowth. Adult OEC/ONF and neonatal astrocytes were cultured either on biodegradable poly(D,L)-lactide matrices or in Petri dishes for 4 days. Thereafter neonatal cerebral cortical neurons were added. After a 2-days coculture period the cultures were fixed and processed for a combined MAP-2 and phosphorylated neurofilament (RT97) staining. The neurite growth (neurite elongation and neurite formation) and the neurite direction were assessed. We show that (1) OEC/ONF cultures are more potent in stimulating the length of the longest neurite of cocultured neurons, (2) alignment of glial is achieved in vitro on our biomatrices, (3) aligned glial/biomatrix complexes do not enhance neurite growth, and (4) aligned glial/biomatrix complexes direct neurite outgrowth. These data have significant implications for in vivo experiments focusing on glial transplantation. Transplanting glial/biomatrix complexes may stimulate the directional regrowth of severed axons across a lesion site. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.
Research Center/Unit :
Center for Education and Research on Macromolecules (CERM)
Deumens, R.; University of Maastricht, The Netherlands > Department of Psychiatry and Neuropsychology > Div. Cellular Neuroscience, European Graduate School for Neuroscience (EURON
Koopmans, G. C.; University of Maastricht, The Netherlands > Department of Psychiatry and Neuropsychology > Div. Cellular Neuroscience, European Graduate School for Neuroscience (EURON)
Den Bakker, C. G. J.; University of Maastricht, The Netherlands > Department of Psychiatry and Neuropsychology > Div. Cellular Neuroscience, European Graduate School for Neuroscience (EURON)
Maquet, V.
Blacher, Silvia ; Université de Liège - ULiège > Department of Applied Chemistry > Laboratory of Chemical Engineering
Honig, W. M. M.; University of Maastricht and Academic Hospital Maastricht, The Netherlands > Department of Anesthesiology (Hospital), Department of Psychiatry and Neuropsychology (University) > Div. Cellular Neuroscience, European Graduate School for Neuroscience (EURON)
Jérôme, Robert ; Université de Liège - ULiège > Département de chimie (sciences) > Département de chimie (sciences)
Pirard, Jean-Paul ; Université de Liège - ULiège > Department of Applied Chemistry > Laboratory of Chemical Engineering
Steinbusch, H. W. M.; University of Maastricht and Academic Hospital Maastricht, The Netherlands > Department of Anesthesiology (Hospital), Department of Psychiatry and Neuropsychology (University) > Div. Cellular Neuroscience, European Graduate School for Neuroscience (EURON)
Joosten, E. A. J.; University of Maastricht and Academic Hospital Maastricht, The Netherlands > Department of Anesthesiology (Hospital), Department of Psychiatry and Neuropsychology (University) > Div. Cellular Neuroscience, European Graduate School for Neuroscience (EURON)
Language :
English
Title :
Alignment of glial cells stimulates directional neurite growth of cns neurons in vitro
Publication date :
2004
Journal title :
Neuroscience
ISSN :
0306-4522
eISSN :
1873-7544
Publisher :
Elsevier Science, New York, United States - New York
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