Article (Scientific journals)
Evaluation of the anesthetic-lipid association constant. A monolayer approach.
Guilmin, T.; Goormaghtigh, E.; Brasseur, Robert et al.
1982In Biochimica et Biophysica Acta, 685 (2), p. 169-76
Peer Reviewed verified by ORBi
 

Files


Full Text
5-cv.pdf
Publisher postprint (493.72 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
4-Aminobenzoic Acid; Cardiolipins; Liposomes; Mathematics; Membrane Potentials; Phosphatidylserines; Phospholipids; Procaine; Pulmonary Surfactants; Tetracaine
Abstract :
[en] A new approach is presented which allows to describe the binding of different local anesthetics to lipids. Lipids (DL- alpha-dipalmitoylphosphatidylcholine, phosphatidylserine, cardiolipin) are spread at the air-water interface and the anesthetic (procaine, butacaine, tetracaine) injected into the aqueous subphase. The equilibrium constants associated to the interfacial reaction: D+ (subphase) +L- (monolayer) in equilibrium DL (monolayer) (where D+ denotes the anesthetics, L- the lipid anionic site and DL the complex) are calculated from an experimental evaluation of the surface potential of the lipid monolayer. This mode of determination is based essentially on the good correlation between the experimental values of the surface potential and the theoretical predictions from the Gouy-Chapman theory. Fluorescence measurements on liposomes are carried out in order to locate the position of the drug in the lipid layer. This method can be extended to any positively charged drug-anionic lipid interaction.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Guilmin, T.
Goormaghtigh, E.
Brasseur, Robert ;  Université de Liège - ULiège > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biophysique moléc. numér.
Caspers, C.
Ruysschaert, J. M.
Language :
English
Title :
Evaluation of the anesthetic-lipid association constant. A monolayer approach.
Publication date :
1982
Journal title :
Biochimica et Biophysica Acta
ISSN :
0006-3002
eISSN :
1878-2434
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
685
Issue :
2
Pages :
169-76
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 January 2012

Statistics


Number of views
48 (1 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
23
Scopus citations®
without self-citations
17
OpenCitations
 
22

Bibliography


Similar publications



Contact ORBi