Assessment of pregnancy-associated glycoprotein (PAG) concentrations in swamp buffalo samples from fetal and maternal origins by using interspecies antisera.

Nguyen, V. H.; Barbato, O.; Bui, X. N.; Beckers, Jean-François; Melo de Sousa, Noelita

doi:10.1111/j.1740-0929.2012.01009.x

Article (Scientific journals)

Assessment of pregnancy-associated glycoprotein (PAG) concentrations in swamp buffalo samples from fetal and maternal origins by using interspecies antisera.

Nguyen, V. H.; Barbato, O.; Bui, X. N. et al.

2012 • In Animal Science Journal, 83 (10), p. 683-689

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/106267

DOI
10.1111/j.1740-0929.2012.01009.x

PubMed
23035707

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Keywords :

Swamp buffalo; Pregnancy-associated glycoproteins; Maternal plasma; Fetal plasma; Interspecies antisera

Abstract :

[en] Pregnancy-associated glycoproteins (PAG) constitute a large family of glycoproteins found in the outer placental epithelial cell layer of the placenta in Eutherian species. In ruminants, they are noted to be structurally closely related among the different species. This study was designed to determine PAG concentrations in maternal and fetal plasma, allantoic and amniotic fluids in buffalo species. Antisera (AS) generated in rabbits against distinct PAG molecules were used in three RIA-PAG systems: RIA-1 (raised against bovine PAG67kDa; AS#497), RIA-2 (raised against caprine PAG55+62kDa; AS#706) or RIA-3 (raised against buffalo PAG; AS#859). Samples were collected at slaughterhouse (n = 67). PAG concentrations determined by RIA-2 gave significantly higher results in both allantoic and amniotic fluids (12.7 ± 2.1 ng/ml and 24.0 ± 7.3 ng/ml, respectively). Regarding maternal and fetal plasma, PAG concentrations obtained by RIA-2 (21.8 ± 2.4 ng/ml and 20.2 ± 2.5 ng/ml, respectively) and RIA-3 (25.0 ± 2.2 ng/mL and 21.9 ± 3.2 ng/ml, respectively) were higher than those obtained by RIA-1 (15.5 ± 1.4 ng/ml and 16.1 ± 1.8 ng/ml, respectively). The correlation among the three systems was very high. The study clearly reveals the ability of PAG-RIA systems to determine PAG concentration in swamp buffalo samples.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Nguyen, V. H.

Barbato, O.

Bui, X. N.

Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Language :

English

Title :

Assessment of pregnancy-associated glycoprotein (PAG) concentrations in swamp buffalo samples from fetal and maternal origins by using interspecies antisera.

Publication date :

October 2012

Journal title :

Animal Science Journal

ISSN :

1344-3941

eISSN :

1740-0929

Publisher :

Blackwell Publishing, Japan

Volume :

Issue :

Pages :

683-689

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

BTC - Belgian Technical Cooperation
F.R.S.-FNRS - Fonds de la Recherche Scientifique
Walloon region

Funding text :

This study was supported by grants from Belgian Technical Cooperation (BTC) to N. Van Hanh, and from FNRS (Grant number CC 1.5.059.08) and Ministry of the Walloon Region-DGA (Grant number D31-1184) to J.F. Beckers. We thank Dr J. Sulon for help and advice with the RIA procedures and Mrs. R. Fares- Noucairi for her editorial assistance.

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