Reference : Induction of Sparc by Vegf in Human Vascular Endothelial Cells
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/10443
Induction of Sparc by Vegf in Human Vascular Endothelial Cells
English
Kato, Y. [> > > >]
Lewalle, J. M. [> > > >]
Baba, Y. [> > > >]
Tsukuda, M. [> > > >]
Sakai, N. [> > > >]
Baba, M. [> > > >]
Kobayashi, K. [> > > >]
Koshika, S. [> > > >]
Nagashima, Y. [> > > >]
Frankenne, Francis [> >]
Noël, Agnès mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme >]
Foidart, Jean-Michel mailto [Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique >]
Hata, R. I. [> > > >]
21-Sep-2001
Biochemical and Biophysical Research Communications
Academic Press
287
2
422-6
Yes (verified by ORBi)
International
0006-291X
1090-2104
San Diego
CA
[en] SPARC/osteonectin/BM-40 is a matricellular protein that is thought to be involved in angiogenesis and endothelial barrier function. Previously, we have detected high levels of SPARC expression in endothelial cells (ECs) adjacent to carcinomas of kidney and tongue. Although SPARC-derived peptide showed an angiogenic effect, intact SPARC itself inhibited the mitogenic activity of vascular endothelial growth factor (VEGF) for ECs by the inhibiting phosphorylation of flt-1 (VEGF receptor 1) and subsequent ERK activation. Thus, the role of SPARC in tumor angiogenesis, stimulation or inhibition, is still unclear. To clarify the role of SPARC in tumor growth and progression, we determined the effect of VEGF on the expression of SPARC in human microvascular EC line, HMEC-1, and human umbilical vein ECs. VEGF increased the levels of SPARC protein and steady-state levels of SPARC mRNA in serum-starved HMEC-1 cells. Inhibitors (SB202190 and SB203580) of p38, a mitogen-activated protein (MAP) kinase, attenuated VEGF-stimulated SPARC production in ECs. Since intact SPARC inhibits phosphorylation ERK MAP kinase in VEGF signaling, it was suggested that SPARC plays a dual role in the VEGF functions, tumor angiogenesis, and extravasation of tumors mediated by the increased permeability of endothelial barrier function.
http://hdl.handle.net/2268/10443
10.1006/bbrc.2001.5622

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