Article (Scientific journals)
Reduction of B cell turnover in chronic lymphocytic leukaemia.
Defoiche, Julien; Debacq, Christophe; Asquith, Becca et al.
2008In British Journal of Haematology, 143 (2), p. 240-7
Peer Reviewed verified by ORBi


Full Text
Defoiche BJH final.pdf
Publisher postprint (186.64 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to


Keywords :
Adult; Aged; B-Lymphocytes/metabolism/pathology; Case-Control Studies; Cell Proliferation; DNA/metabolism; Deoxyadenosines/analysis/metabolism; Deuterium/analysis/diagnostic use/metabolism; Female; Humans; Leukemia, Lymphocytic, Chronic, B-Cell/immunology; Male; Middle Aged
Abstract :
[en] Whether chronic lymphocytic leukaemia (CLL) is a latent or a proliferating disease has been intensively debated. Whilst the dogma that CLL results from accumulation of dormant lymphocytes is supported by the unresponsiveness of leukaemic cells to antigens and polyclonal activators, recent in vivo kinetic measurements indicate that B lymphocytes do divide at significant rates in CLL. However, an important and still unanswered question is whether CLL cells proliferate faster or slower compared with their normal counterparts. This report addressed directly this point and compared B-cell kinetics in CLL subjects and healthy controls, using a pulse-chase approach based on incorporation of deuterium from 6,6-(2)H(2)-glucose into DNA. We confirmed that B cells proliferated at significant levels in CLL but found that the proliferation rates were reduced compared with healthy subjects (mean 0.47 vs. 1.31%/d respectively, P = 0.007), equivalent to an extended doubling time of circulating B cells (147 d vs. 53 d). In conclusion, CLL B cells proliferate at reduced levels compared with healthy controls. CLL is thus characterized by an aberrant B-cell kinetics with a decrease in cell turnover, an observation that may impact on elaboration of efficient therapeutic strategies.
Disciplines :
Author, co-author :
Defoiche, Julien
Debacq, Christophe
Asquith, Becca
Zhang, Yan
Burny, Arsène ;  Université de Liège - ULiège > Agro Biotech Gembloux
Bron, Dominique
Lagneaux, Laurence
Macallan, Derek
Willems, Luc  ;  Université de Liège - ULiège > GIGA-Research
Language :
Title :
Reduction of B cell turnover in chronic lymphocytic leukaemia.
Publication date :
Journal title :
British Journal of Haematology
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
Issue :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 April 2009


Number of views
99 (8 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
Scopus citations®
without self-citations


Similar publications

Contact ORBi