Article (Scientific journals)
Integrated backscatter for the in vivo quantification of supraphysiological vitamin D(3)-induced cardiovascular calcifications in rats.
Roosens, Bram; Droogmans, Steven; Hostens, Jeroen et al.
2011In Cardiovascular Toxicology, 11 (3), p. 244-52
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Abstract :
[en] Cardiovascular calcifications are frequently found in the aging population and are independent predictors of future cardiovascular events. Integrated backscatter (IB) of ultrasound reflectivity can easily quantify calcifications. For this purpose, 30 male Wistar rats received 25,000 IU/kg/day of vitamin D(3) (group 1, n = 8), 18,800 IU/kg/day (group 2, n = 8), or injections with the vehicle only (group 3, n = 14), for 10 weeks. Echocardiographic calibrated IB (cIB) was measured and calculated at baseline and after 10 weeks, followed by ex vivo micro-CT and histopathology of the aortic valve, ascending aorta, and myocardium. After 10 weeks, the mean cIB value of the aortic valve was significantly higher for vitamin D(3)-dosed animals compared to controls. The mean cIB value of the ascending aorta and the myocardium was also significantly higher in group 1 compared to group 3. In vivo IB results were confirmed by ex vivo micro-CT and histopathology. In conclusion, IB is a non-ionizing, feasible, and reproducible tool to quantify cardiovascular calcifications in an in vivo rat model. The integration of IB in the standard echocardiographic examination for the quantification of cardiovascular calcifications could be useful for serial evaluation of treatment efficacy and for prognosis assessment.
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
Roosens, Bram
Droogmans, Steven
Hostens, Jeroen
Somja, Joan ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Delvenne, Eléonore 
Hernot, Sophie
Bala, Gezim
Degaillier, Celine
Caveliers, Vicky
Delvenne, Philippe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Lahoutte, Tony
Van Camp, Guy
Cosyns, Bernard
More authors (3 more) Less
Language :
English
Title :
Integrated backscatter for the in vivo quantification of supraphysiological vitamin D(3)-induced cardiovascular calcifications in rats.
Publication date :
2011
Journal title :
Cardiovascular Toxicology
ISSN :
1530-7905
eISSN :
1559-0259
Publisher :
Humana Press, United States
Volume :
11
Issue :
3
Pages :
244-52
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 November 2011

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