Article (Scientific journals)
Molecular Polymorphisms in Tunisian Pomegranate (Punica granatum L.) as Revealed by RAPD Fingerprints
Hasnaoui, Nejib; Mars, Messaoud; Chibani, Jemni et al.
2010In Diversity, 2 (1), p. 107-114
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Keywords :
Pomegranate; Punica granatum L.; Diversity; RAPD
Abstract :
[en] The genetic diversity among Tunisian pomegranate cultivars has been investigated. Using universal primers, the random amplified polymorphic DNA (RAPD) method was used to generate banding profiles from a set of twelve cultivars. Data was then computed with appropriate programs to construct a dendrogram illustrating the relationships between the studied cultivars. Our data proved the efficiency of the designed method to examine the DNA polymorphism in this crop since the tested primers are characterized by a collective resolving power of 12.83. In addition, the cluster analysis has exhibited a parsimonious tree branching independent from the geographic origin of the cultivars. In spite of the relatively low number of primers and cultivars, RAPD constitutes an appropriate procedure to assess the genetic diversity and to survey the phylogenetic relationships in this crop.
Disciplines :
Agriculture & agronomy
Author, co-author :
Hasnaoui, Nejib ;  Higher Agronomic Institute, 4042 Chott Mariem, Sousse, Tunisia > U.R. Agro-biodiversity
Mars, Messaoud;  Higher Agronomic Institute, 4042 Chott Mariem, Sousse, Tunisia > U.R. Agro-biodiversity
Chibani, Jemni;  Faculty of Pharmacy, 5019 Monastir, Tunisia > Laboratory of Biochemistry & Molecular Biology
Trifi, Mokhtar;  Faculty of Sciences of Tunis, Campus University, 2092, El Manar, Tunis, Tunisia > Laboratory of Molecular Genetics, Immunology & Biotechnology
Language :
English
Title :
Molecular Polymorphisms in Tunisian Pomegranate (Punica granatum L.) as Revealed by RAPD Fingerprints
Publication date :
18 January 2010
Journal title :
Diversity
eISSN :
1424-2818
Publisher :
MDPI AG, Base, Switzerland
Special issue title :
Biological Diversity Assessed by Molecular Methods
Volume :
2
Issue :
1
Pages :
107-114
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 November 2011

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