[en] The genetic diversity among Tunisian pomegranate cultivars has been investigated. Using universal primers, the random amplified polymorphic DNA (RAPD) method was used to generate banding profiles from a set of twelve cultivars. Data was then computed with appropriate programs to construct a dendrogram illustrating the relationships between the studied cultivars. Our data proved the efficiency of the designed method to examine the DNA polymorphism in this crop since the tested primers are characterized by a collective resolving power of 12.83. In addition, the cluster analysis has exhibited a parsimonious tree branching independent from the geographic origin of the cultivars. In spite of the relatively low number of primers and cultivars, RAPD constitutes an appropriate procedure to assess the genetic diversity and to survey the phylogenetic relationships in this crop.
Evreinoff, V.A. Le grenadier. Fruits d'Outre-Mer 1949, 4, 161-170.
Tous, J.; Ferguson L. Mediterranean fruits. In Progress in New Crops, Janick, J., Ed.; ASHS Press: Arlington, VA, USA, 1996; pp. 416-430.
Mars, M. Ressources génétiques du grenadier (Punica granatum L.) en Tunisie: prospection, conservation et analyse de la diversité. Thèse de Doctorat d'Etat, Université El Manar, Faculté des Sciences de Tunis: Tunis, Tunisia, 2001.
Mars, M.; Marrakchi, M. Conservation et valorisation des ressources génétiques du grenadier (Punica granatum L.) en Tunisie. Plant Genet. Resour. Newslett. 1998, 114, 35-39.
Mars, M.; Marrakchi, M. Diversity of pomegranate (Punica granatum L.) germplasm in Tunisia. Genet. Resour. Crop Ev. 1999, 46, 461-467.
Baht, K.V.; Jarret, R.L.; Rana, P.S. DNA profiling of banana and plantain cultivars using random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers. Electrophoresis 1995, 16, 1736-1745.
Onguso, J.M.; Kahangi, E.M.; Ndiritu, D.W.; Mizutani, F. Genetic characterisation of cultivated bananas and plantains in Kenya by RAPD markers. Sci. Hort. 1999, 99, 9-20.
Sarkhosh, A.; Zamani, Z.; Fatahi, R.; Ebadi, A. RAPD markers reveal polymorphism among some Iranian pomegranate (Punica granatum L.) genotypes. Sci. Hort. 2006, 111, 24-29.
Durgaç, C.; Ozgen, M.; Simsek, O.; Kaçar, Y.K.; Kyga, Y.; Celebi, S.; Gunduz, K.; Serce, S. Molecular and pomological diversity among pomegranate (Punica granatum L.) cultivars in Eastern Mediterranean region of Turkey. Afr. J. Biotechnol. 2008, 7, 1294-1301.
Sheidai, M.; Saneghi, A.; Shahreiyari, Z.H.; Noormohammadi, Z.; Farahanei F.; Tabatabaei-Ardakanei, S. RAPD and cytogenetic study of some pomegranate (Punica granatum L.) cultivars. Caryologia 2008, 6, 68-73.
Yuan, Z.; Yin, Y.; Qu, J.; Zhu, L.; Li, Y. Population genetic diversity in Chinese pomegranate (Punica granatum L.) cultivars revealed by fluorescent-AFLP markers. J. Genet. Genomics 2007, 34, 1061-1071.
Jbir, R.; Hasnaoui, N.; Mars, M.; Marrakchi, M.; Trifi, M. Characterization of Tunisian pomegranate (Punica granatum L.) cultivars using amplified fragment length polymorphism analysis. Sci. Hort. 2008, 115, 231-237.
Landry, B.; Li, R.; Cheung, W.; Granger, R. Phylogeny analysis of 25 apple root stocks using RAPD markers and tactical gene targging. Theor. Appl. Genet. 1994, 89, 847-852.
Qu, X.P.; Lu, J.; Lamikanra, O. Genetic diversity in Muscadine and American bunch grapes based on randomly amplified polymorphic DNA (RAPD) analysis. J. Am. Soc. Hort. Sci. 1996, 121, 1020-1023.
Ronning, C.M.; Schnell, R.J.; Gazit, S. Use of random amplified polymorphic DNA (RAPDs) to identify annona cultivars. J. Am. Soc. Hort. Sci. 1995, 120, 726-729.
Ben Abdallah, A.; Stiti, K.; Lepoivre, P.; Du Jardin, P. Identification de cultivars de palmier dattier (Phoenix dactylifera L.) par l'amplification aléatoire d'ADN (RAPD). Cah. Étud. Recher. Francoph./Agric. 2000, 9, 103-107.
Chatti, K.; Salhi-Hannachi, A.; Mars, M.; Marrakchi, M.; Trifi, M. Genetic diversity and phylogenic relationships in Tunisian fig (Ficus carica L.) cultivars mediated by RAPD. Biol. Tunis. 2004, 1, 1-4.
Ebrahimi, R.; Zamani, Z.; Kashi, A. Genetic diversity evaluation of wild Persian shallot (Allium hirtifolium Boiss.) using morphological and RAPD markers. Sci. Hort. 2009, 119, 345-351.
Salhi-Hannachi, A.; Chatti, K.; Mars, M.; Marrakchi, M.; Trifi, M. Comparative analysis of genetic diversity in two collections of fig cultivars based on random amplified polymorphic DNA and inter simple sequence repeats fingerprints. Genet. Resour. Crop Ev. 2005, 52, 563-573.
Dellaporta, S.L.; Wood, J.; Hicks, J.B. A plant DNA minipreparation: version II. Plant Mol. Biol. Rep. 1983, 1, 19-21.
Sambrook, J.; Fritsch, E.F.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: New York, NY, USA, 1989.
Williams, J.G.K.; Kubelik, A.R.; Livak, J.; Rafalski, J.A.; Tingey, S.V. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990, 18, 6531-6535.
Gilbert, J.E.; Lewis, E.V.; Wilkinson, M.J.; Caligari, P.D.S. Developing an appropriate strategy to assess genetic variability in plant germplasm collections. Theor. Appl. Genet. 1999, 98, 1125-1131.
Nei, M.; Li, W.H. Mathematical models for studying genetic variation in terms of restriction endonucleases. P. Natl. Acad. Sci. USA 1979, 76, 5269-5273.
Felsenstein, J. PHYLIP (Phylogeny Interference Package) version 3,5 c; Department of Genetics, University of Washington: Seattle, WA, USA, 1995.
Page, R.D.M. Tree View: An application to display phylogenetic trees on personal computers. Comput. Appl. Biosci. 1996, 12, 357-358.
