Article (Scientific journals)
Effects of exogenous IL-1 beta, TNF alpha, IL-6, IL-8 and LIF on cytokine production by human articular chondrocytes.
Henrotin, Yves; De Groote, D. D.; Labasse, A. H. et al.
1996In Osteoarthritis and Cartilage, 4 (3), p. 163-73
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Keywords :
Cartilage, Articular/cytology/drug effects/metabolism; Cells, Cultured; Cytokines/biosynthesis/drug effects; Growth Inhibitors/pharmacology; Humans; Immunoenzyme Techniques; Interleukin-1/pharmacology; Interleukin-6/pharmacology; Interleukin-8/pharmacology; Interleukins/pharmacology; Leukemia Inhibitory Factor; Lymphokines/pharmacology; Recombinant Proteins; Tumor Necrosis Factor-alpha/pharmacology
Abstract :
[en] Cytokines are potent regulators of the chondrocyte functions. Some of them are produced by chondrocytes and interact to regulate cartilage metabolism. In this study, we investigated the production of interleukin-1 beta (IL-1 beta), IL-6, IL-8 and leukemia inhibitory factor (LIF) by human chondrocytes and examined the modulation of their secretion by exogenous cytokines. Human articular chondrocytes were isolated from their extracellular matrix by a triple successive enzymatic digestion of the cartilage. Subsequently, chondrocytes were stimulated by increased amounts of human recombinant cytokines [IL-1 beta, tumour necrosis factor alpha (TNF alpha), IL-8, LIF, IL-6]. IL-1 beta, IL-6, IL-8 and LIF were assayed into culture media and inside cell extracts by specific enzyme amplified sensitivity immunoassays (EASIAs). Under these experimental conditions, we have identified various interactions between cytokines. IL-beta and TNF alpha highly stimulated IL-6, LIF and IL-8 productions. IL-6 decreased IL-8 synthesis and increased LIF production. IL-8 slightly enhanced IL-6 production. Finally, LIF stimulated IL-1 beta, IL-6 and IL-8 productions. Using neutralizing antibodies against IL-1, we demonstrated that the effects of LIF were secondary to the stimulation by LIF of IL-1 beta production by the chondrocytes. In conclusion, chondrocytes secrete a variety of immunocompetent cytokines including IL-1 beta, IL-6, IL-8 and LIF that can interact to regulate chondrocytes metabolism. These results also define new biological activities of LIF and IL-6, and raise questions concerning their role in the pathogenesis of joint diseases.
Disciplines :
Rheumatology
Author, co-author :
Henrotin, Yves  ;  Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.)
De Groote, D. D.
Labasse, A. H.
Gaspar, S. E.
Zheng, S. X.
Geenen, Vincent ;  Université de Liège - ULiège > Centre d'immunologie
Reginster, Jean-Yves  ;  Université de Liège - ULiège > Département des sciences de la santé publique > Santé publique, Epidémiologie et Economie de la santé
Language :
English
Title :
Effects of exogenous IL-1 beta, TNF alpha, IL-6, IL-8 and LIF on cytokine production by human articular chondrocytes.
Publication date :
1996
Journal title :
Osteoarthritis and Cartilage
ISSN :
1063-4584
eISSN :
1522-9653
Publisher :
W.B. Saunders, London, United Kingdom
Volume :
4
Issue :
3
Pages :
163-73
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 November 2011

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