Reference : Production of active oxygen species by isolated human chondrocytes.
Scientific journals : Article
Human health sciences : Rheumatology
http://hdl.handle.net/2268/103547
Production of active oxygen species by isolated human chondrocytes.
English
Henrotin, Yves mailto [Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.)]
Deby-Dupont, G. [> > > >]
DEBY, C. [ > > ]
De Bruyn, M. [ > > ]
Lamy, Maurice mailto [Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques >]
FRANCHIMONT, P. [ > > ]
1993
British Journal of Rheumatology
Oxford University Press
32
7
562-7
Yes (verified by ORBi)
International
0263-7103
Oxford
United Kingdom
[en] Adolescent ; Adult ; Aged ; Aged, 80 and over ; Anoxia/metabolism/physiopathology ; Cartilage/cytology/metabolism ; Cells, Cultured ; Ethylenes/metabolism ; Female ; Flow Cytometry ; Free Radicals/metabolism ; Humans ; Hydrogen Peroxide/metabolism ; Hydroxides/metabolism ; Hydroxyl Radical ; Male ; Methionine/analogs & derivatives/metabolism ; Middle Aged ; Reactive Oxygen Species/metabolism ; Tetradecanoylphorbol Acetate/pharmacology
[en] The ability of isolated human chondrocytes to produce active oxygen species has been investigated. The two methods for determining H2O2 and hydroxyl radicals (.OH) production were, by a fluorimetric method (production of dichlorofluorescein from a precursor in the presence of horseradish peroxidase and H2O2) and by a chromatographic method (measurement of ethylene production from gamma-methiol-keto-butyric acid after .OH attack). Chondrocytes were tested, both with and without activation by phorbol myristate acetate (PMA: 10(-6) M), in the presence of Ca2+ (1 x 10(-4) M) and Mg2+ (2 x 10(-4) M) or after variable periods of anoxia under nitrogen (4 to 12 h) followed by reoxygenation (with 95% O2, 5% CO2). Under these experimental conditions, the PMA-excited chondrocytes produced from 80 to 180 nmol of hydrogen peroxide per 1 x 10(6) cells and chondrocytes subjected to anoxia-reoxygenation produced up to 1700 nmol H2O2 per 1 x 10(6) cells. The hydroxyl radical production by PMA or anoxia-reoxygenation excited cells reached 600% of the production of non-excited cells and 1300% when they were subjected to successive stimulations by PMA and anoxia-reoxygenation. The possible pathological significance of these observations is discussed. The results indicate that stimulated human chondrocytes are capable of producing active oxygen species which could play a major role in joint inflammation and cartilage damage.
http://hdl.handle.net/2268/103547

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