Relationship between arthroscopic joint evaluation and the levels of Coll2-1, Coll2-1NO(2), and myeloperoxidase in the blood and synovial fluid of horses affected with osteochondrosis of the tarsocrural joint.
[en] OBJECTIVE: To evaluate the levels of plasmatic and synovial Coll2-1, Coll2-1NO(2) and myeloperoxidase (MPO) in horses with osteochondral lesions of the tarsocrural joint and to investigate how these levels relate to arthroscopic findings of inflammation and degeneration. MATERIALS AND METHODS: Venous blood and synovial fluid samples were collected from 63 horses presented for arthroscopic removal of osteochondral fragments in the tarsocrural joint. Prior to removal of the osteochondral fragment, an exploration of the joint was performed and an inflammatory and degenerative score was determined. The blood and synovial levels of Coll2-1, Coll2-1NO(2) and MPO were also measured. The effects of the arthroscopic evaluation (inflammatory and degenerative classes) on the blood and synovial markers were evaluated using a linear model (GLM procedure), and correlations between biochemical markers in the blood and synovial fluid and the arthroscopic evaluation (inflammatory and degenerative classes) were established (Pearson's correlations). RESULTS: Significantly higher levels of Coll2-1 were detected in synovial fluid of higher degenerative classes. There was a significant correlation between the degenerative score and the synovial levels of Coll2-1 (r=0.27). According to the logistic regression model, there was a significant effect of the degenerative class on synovial levels of Coll2-1. CONCLUSIONS: Coll2-1 correlates well with the degenerative state of tarsocrural joints as evaluated by arthroscopy. This marker can therefore be classified as a burden-of-disease marker in the assessment of joint disease in horses.
Disciplines :
Rheumatology
Author, co-author :
Verwilghen, Denis ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Enzerink, E.
Martens, A.
Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Balligand, Marc ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Chirurgie et clinique chirurgicale des petits animaux
Henrotin, Yves ; Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.)
Detilleux, Johann ; Université de Liège - ULiège > Département de productions animales > Génétique quantitative
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Relationship between arthroscopic joint evaluation and the levels of Coll2-1, Coll2-1NO(2), and myeloperoxidase in the blood and synovial fluid of horses affected with osteochondrosis of the tarsocrural joint.
Publication date :
2011
Journal title :
Osteoarthritis and Cartilage
ISSN :
1063-4584
eISSN :
1522-9653
Publisher :
W.B. Saunders, London, United Kingdom
Volume :
19
Issue :
11
Pages :
1323-9
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2011 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
McIlwraith C.W. Developmental orthopedic disease: problems of limbs in young horses. J Equine Vet Sci 2004, 24:475-479.
Runge J.J., Biery D.N., Lawler D.F., Gregor T.P., Evans R.H., Kealy R.D., et al. The effects of lifetime food restriction on the development of osteoarthritis in the canine shoulder. Vet Surg 2008, 37:102-107.
Van Weeren P.R. Osteochondrosis. Equine Surgery 2006, 1166-1178. Elsevier Saunders, St. Louis, Mo. J.A. Auer, J.A. Stick (Eds.).
de Grauw J.C., Brama P.A., Wiemer P., Brommer H., van de Lest C.H., van Weeren P.R. Cartilage-derived biomarkers and lipid mediators of inflammation in horses with osteochondritis dissecans of the distal intermediate ridge of the tibia. Am J Vet Res 2006, 67:1156-1162.
Donabedian M., van Weeren P.R., Perona G., Fleurance G., Robert C., Leger S., et al. Early changes in biomarkers of skeletal metabolism and their association to the occurrence of osteochondrosis (OC) in the horse. Equine Vet J 2008, 40:253-259.
Fuller C.J., Barr A.R., Sharif M., Dieppe P.A. Cross-sectional comparison of synovial fluid biochemical markers in equine osteoarthritis and the correlation of these markers with articular cartilage damage. Osteoarthritis Cartilage 2001, 9:49-55.
McIlwraith C.W. Use of synovial fluid and serum biomarkers in equine bone and joint disease: a review. Equine Vet J 2005, 37:473-482.
Trumble T.N., Scarbrough A.B., Brown M.P. Osteochondral injury increases type II collagen degradation products (C2C) in synovial fluid of Thoroughbred racehorses. Osteoarthritis Cartilage 2009, 17:371-374.
Verwilghen D., Busoni V., Gangl M., Franck T., Lejeune J.P., Vanderheyden L., et al. Relationship between biochemical markers and radiographic scores in the evaluation of the osteoarticular status of Warmblood stallions. Res Vet Sci 2009, 87:319-328.
Wang Y.X., Griffith J.F., Ahuja A.T. Non-invasive MRI assessment of the articular cartilage in clinical studies and experimental settings. World J Radiol 2010, 2:44-54.
Bouchgua M., Verwilghen D.R., Busoni V. Ultrasonographic evaluation of the articular cartilage of the dorsal metacarpal/tarsal condyle in the horse. EVDI Annual Meeting. Svolvaer, Norway 2008.
Gangl M., Serteyn D., Lejeun J.P., Schneider N., Grulke S., Peters F., et al. A type II-collagen derived peptide and its nitrated form as new markers of inflammation and cartilage degradation in equine osteochondral lesions. Res Vet Sci 2007, 82:68-75.
Lejeune J.P., Serteyn D., Gangl M., Schneider N., Deby-Dupont G., Deberg M., et al. Plasma concentrations of a type II collagen-derived peptide and its nitrated form in growing Ardenner sound horses and in horses suffering from juvenile digital degenerative osteoarthropathy. Vet Res Commun 2007, 31:591-601.
Deberg M., Labasse A., Christgau S., Cloos P., Henriksen D.B., Chapelle J.P., et al. New serum biochemical markers (Coll 2-1 and Coll 2-1 NO2) for studying oxidative-related type II collagen network degradation in patients with osteoarthritis and rheumatoid arthritis. Osteoarthritis Cartilage 2005, 13:258-265.
Deberg M.A., Labasse A.H., Collette J., Seidel L., Reginster J.Y., Henrotin Y.E. One-year increase of Coll 2-1, a new marker of type II collagen degradation, in urine is highly predictive of radiological OA progression. Osteoarthritis Cartilage 2005, 13:1059-1065.
Henrotin Y., Deberg M., Dubuc J.E., Quettier E., Christgau S., Reginster J.Y. Type II collagen peptides for measuring cartilage degradation. Biorheology 2004, 41:543-547.
Beckman J.S., Koppenol W.H. Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and the ugly. Am J Physiol Cell Physiol 1996, 40:C1424-C1437.
Henrotin Y., Debydupont G., Deby C., Debruyn M., Lamy M., Franchimont P. Production of active oxygen species by isolated human chondrocytes. Br J Rheumatol 1993, 32:562-567.
Henrotin Y.E., Zheng S.X., Deby G.P., Labasse A.H., Crielaard J.M.R., Reginster J.Y.L. Nitric oxide downregulates interleukin 1 beta (IL-1 beta) stimulated IL-6, IL-8, and prostaglandin E-2 production by human chondrocytes. J Rheumatol 1998, 25:1595-1601.
Schneider N., Mouithys-Mickalad A.L., Lejeune J.P., Deby-Dupont G.P., Hoebeke M., Serteyn D.A. Synoviocytes, not chondrocytes, release free radicals after cycles of anoxia/re-oxygenation. Biochem Biophys Res Comm 2005, 334:669-673.
Kaur H., Halliwell B. Evidence for nitric oxide-mediated oxidative damage in chronic inflammation - nitrotyrosine in serum and synovial-fluid from rheumatoid patients. FEBS Letters 1994, 350:9-12.
Baldus S., Eiserich J.P., Brennan M.L., Jackson R.M., Alexander C.B., Freeman B.A. Spatial mapping of pulmonary and vascular nitrotyrosine reveals the pivotal role of myeloperoxidase as a catalyst for tyrosine nitration in inflammatory diseases. Free Radic Biol Med 2002, 33:1010-1019.
Brennan M.L., Wu W.J., Fu X.M., Shen Z.Z., Song W., Frost H., et al. A tale of two controversies - defining both the role of peroxidases in nitrotyrosine formation in vivo using eosinophil peroxidase and myeloperoxidase-deficient mice, and the nature of peroxidase-generated reactive nitrogen species. J Biol Chem 2002, 277:17415-17427.
Burner U., Furtmuller P.G., Kettle A.J., Koppenol W.H., Obinger C. Mechanism of reaction of myeloperoxidase with nitrite. J Biol Chem 2000, 275:20597-20601.
Serteyn D., Grulke S., Franck T., Mouithys-Mickalad A., Deby-Dupont G. Neutrophil myeloperoxidase, protective enzyme with strong oxidative activities. Ann Med Vet 2003, 147:79-93.
Deberg M., Dubuc J.E., Labasse A., Sanchez C., Quettier E., Bosseloir A., et al. One year follow-up of Coll2-1, Coll2-1NO2 and myeloperoxydase serum levels in OA patients after hip or knee replacement. Ann Rheum Dis 2008, 67:168-174.
Kuwabara K., Jyoyama H., Fleisch J.H., Hori Y. Inhibition of antigen-induced arthritis in guinea pigs by a selective LTB4 receptor antagonist LY293111Na. Inflamm Res 2002, 51:541-550.
Verwilghen DR, Martens A, Busschers E, Franck T, Deberg M, Henrotin Y, et al. Coll2-1, Coll2-1NO2 and myeloperoxidase concentrations in the synovial fluid of equine tarsocrural joints affected with osteochondrosis. Vet Res Comm in press, doi:10.1007/s11259-011-9487-5.
McIlwraith C.W., Wright I.M., Nixon A.J., Boening K.J. Diagnostic and Surgical Arthroscopy in the Horse 2005, Elsevier Limited, Edinburgh; New York. 3rd edn.
Trumble T.N., Brown M.P., Merritt K.A., Billinghurst R.C. Joint dependent concentrations of bone alkaline phosphatase in serum and synovial fluids of horses with osteochondral injury: an analytical and clinical validation. Osteoarthritis Cartilage 2008, 16:779-786.
Ayral X., Dougados M., Listrat V., Bonvarlet J.P., Simonnet J., Amor B. Arthroscopic evaluation of chondropathy in osteoarthritis of the knee. J Rheumatol 1996, 23:698-706.
Franck T., Grulke S., Deby-Dupont G., Deby C., Duvivier H., Peters F., et al. Development of an enzyme-linked immunosorbent assay for specific equine neutrophil myeloperoxidase measurement in blood. J Vet Diagn Investig 2005, 17:412-419.
Garvican E.R., Vaughan-Thomas A., Innes J.F., Clegg P.D. Biomarkers of cartilage turnover. Part 1: markers of collagen degradation and synthesis. Vet J 2010, 185:36-42.
Ameye L.G., Deberg M., Oliveira M., Labasse A., Aeschlimann J.M., Henrotin Y. The chemical biomarkers C2C, Coll2-1, and Coll2-1NO2 provide complementary information on type II collagen catabolism in healthy and osteoarthritic mice. Arthritis Rheum 2007, 56:3336-3346.
Henrotin Y., Addison S., Kraus V., Deberg M. Type II collagen markers in osteoarthritis: what do they indicate?. Curr Opin in Rheumatol 2007, 19:444-450.
Lettry V., Sumie Y., Mitsuda K., Tagami M., Hosoya K., Takagi S., et al. Divergent diagnosis from arthroscopic findings and identification of CPII and C2C for detection of cartilage degradation in horses. Jpn J Vet Res 2010, 57:197-206.
Bauer D.C., Hunter D.J., Abramson S.B., Attur M., Corr M., Felson D., et al. Classification of osteoarthritis biomarkers: a proposed approach. Osteoarthritis Cartilage 2006, 14:723-727.
Fietz S., Bondzio A., Moschos A., Hertsch B., Einspanier R. Measurement of equine myeloperoxidase (MPO) activity in synovial fluid by a modified MPO assay and evaluation of joint diseases - an initial case study. Res Vet Sci 2008, 84:347-353.
Myers S.L., Brandt K.D., Eilam O. Even low-grade synovitis significantly accelerates the clearance of protein from the canine knee. Implications for measurement of synovial fluid "markers" of osteoarthritis. Arthritis Rheum 1995, 38:1085-1091.
Ayral X., Gueguen A., Ike R.W., Bonvarlet J.P., Frizziero L., Kalunian K., et al. Inter-observer reliability of the arthroscopic quantification of chondropathy of the knee. Osteoarthritis Cartilage 1998, 6:160-166.
Oakley S.P., Lassere M.N. A critical appraisal of quantitative arthroscopy as an outcome measure in osteoarthritis of the knee. Semin Arthritis Rheum 2003, 33:83-105.
Beguin J., Locker B. Chondropathie Rotulienne. Deuxieme journée d'arthroscopie du genou 1983, vol. 1:89-90. Lyon, France.
Ayral X., Pickering E.H., Woodworth T.G., Mackillop N., Dougados M. Synovitis: a potential predictive factor of structural progression of medial tibiofemoral knee osteoarthritis - results of a 1 year longitudinal arthroscopic study in 422 patients. Osteoarthritis Cartilage 2005, 13:361-367.
