[en] Objective: To evaluate the implantation of menstrual endometrium and the early stages of evolution of endometriotic lesions.
Design: Experimental prospective study.
Setting: An academic research environment.
Animals: Ten nude mice.
Intervention(s): A minilaparotomy was performed to place fresh human menstrual endometrial samples in the peritoneal cavity. Removal of the transplants was performed successively on days 1, 3, and 5 by laparotomy.
Main Outcome Measure(s): Adhesion of endometrial fragments and early stages of endometrial lesions was morphologically and immunohistochemically studied.
Result(s): As early as day 1, stromal cells were found to be attached to the mesothelium. A progressive reorganization of epithelial and stromal cells into endometrial glands was observed. On day 5, cystic endometriotic lesions were characterized by more extensive proliferative activity in glandular cells and a higher VEGF score in stromal cells than that observed in previously removed transplants.
Conclusion(s): Menstrual human endometrium is able to implant on intact mesothelium and to reorganize itself into structured glands and stroma under the influence of unknown factors. We suggest that stromal and glandular cells have two distinct roles: stromal cells are involved in the attachment process and glandular cells in the growth of the endometriotic lesion.
Disciplines :
Médecine de la reproduction (Gynécologie, andrologie, obstétrique)
Auteur, co-auteur :
NISOLLE, Michelle ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Casanas-Roux, Françoise
Donnez, Jacques
Langue du document :
Anglais
Titre :
Early-stage endometriosis: adhesion and growth of human menstrual endometrium in nude mice
Sampson J. Peritoneal endometriosis is due to the menstrual dissemination of endometrial tissue into the peritoneal cavity. Am J Obstet Gynecol. 14:1927;433-469.
Van der Linden P.J., de Goeij A.F., Dunselman G.A., Van der Linden E.P., Ramackers F.C., Evers J.L. Expression of integrins and E-cadherin in cells from menstrual effluent, endometrium, peritoneal fluid, peritoneum and endometriosis. Fertil Steril. 61:1994;85-90.
Beliard A., Donnez J., Nisolle M., Foidart J.M. Localization of laminin, fibronectin, E-cadherin and integrins in endometrium and endometriosis. Fertil Steril. 67:1997;266-272.
Witz C.A., Monotoya-Rodriguez I.A., Schenken R.S. Whole explants of peritoneum and endometrium a novel model of the early endometriosis lesion . Fertil Steril. 71:1999;56-60.
Van der Linden P.Q.J., de Goeij A.F., Dunselman G.A., Erkens H.W., Evers J.L. Endometrial cell adhesion is an in vitro model using intact amniotic membrane. Fertil Steril. 65:1996;76-80.
Groothuis P.G., Koks C.A., de Goeij A.F., Dunselman G.A., Arend S.J.W., Evers J.L. Adhesion of human endometrium to the epithelial lining and extracellular matrix of amnion in vitro an electron microscopic study . Hum Reprod. 13:1998;2275-2281.
Groothuis P.G., Koks C.A., de Goeij A.F., Dunselman G.A., Arends J.W., Evers J.L. Adhesion of human endometrial fragments to peritoneum in vitro. Fertil Steril. 71:1999;1119-1124.
Nisolle M., Casanas-Roux F., Marbaix E., Jadoul P., Donnez J. Transplantation of cultured explants of human endometrium into nude mice. Hum Reprod. 15:2000;101-106.
Koks C.A., Groothuis P.G., Dunselman G.A., de Goeij A.F., Evers J.L. Adhesion of shed menstrual tissue in an in-vitro model using amnion and peritoneum a light and electron microscopic study . Hum Reprod. 14:1999;816-822.
Zamah N.M., Dodson M.G., Stephens L.C., Buttram V.C., Besch P.K., Kaufman R.H. Transplantation of normal and ectopic human endometrial tissue into athymic nude mice. Am J Obset Gynecol. 149:1984;591-597.
Berqgvist A., Jeppsson S., Kullander S., Ljunberg O. Human endometrium transplanted into nude mice. Histologic effects of various steroid hormones. Am J Pathol. 119:1985;336-344.
Aoki D., Katsuki Y., Shimizu A., Kakinuma E., Nozawa S. Successful heterotransplantation of human endometrium in SCID mice. Obstet Gynecol. 83:1994;220-228.
Bruner K.L., Matrisian L.M., Rodgers W.H., Gorstein F., Osteen K.G. Progesterone suppresion of matrix metalloproteinases prevents establishment of endometriotic-like human lesions in nude mice. J Clin Invest. 99:1997;2851-2857.
Noyes R.W., Hertig A.R., Rock J. Dating the endometrial biopsy. Fertil Steril. 1:1950;3-25.
Gabe M. Les colorations topographiques. Masson et Cie. Techniques histologiques. 1968;203-256 Masson, Paris.
Nisolle M., Casanas-Roux F., Donnez J. Immunohistochemical analysis of proliferative activity and steroid receptor expression in peritoneal and ovarian endometriosis. Fertil Steril. 68:1997;912-917.
Donnez J., Smoes P., Gillerot S., Casanas-Roux F., Nisolle M. Vascular endothelial growth factor (VEGF) in endometriosis. Hum Reprod. 13:1998;1686-1690.
Siegel S. Non parametric statistics for the behavioral sciences. International student edition. 1956;McGraw-Hill, New York.
Zhang R., Wild R.A., Ojago J.M. Effect of tumor necrosis factor-α on adhesion of human endometrial stromal cells to peritoneal mesothelial cells an in vitro system . Fertil Steril. 59:1993;1196-1201.
Wild R.A., Zhang R., Medders D. Whole fragments from characteristics of in vivo endometriosis in a mesothelial cell co-culture system an in vitro model of the histogenesis of endometriosis . J Soc Gynecol Invent. 1:1994;165-168.
Singer C., Marbaix E., Kokorine I., Leaoine P., Donnez J., Eeckhout Y., Courtoy P.J. Paracrine stimulation of intersitial collagenase (MMP-1) in the human endometrium by interleukin 1a and its dual block by ovarian steroids. Proc Natl Acad Sci USA. 94:1997;10341-10345.
