Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study.
Van Langendonckt, Anne; Demylle, Dominique; Wyns, Christineet al.
2001 • In Fertility and Sterility, 76 (5), p. 1023-31
Human embryo culture; sequential culture medium; IVF; blastocyst; implantation rate
Abstract :
[en] Objective: To compare two commercially available sequential media, G1.2/G2.2 and Sydney IVF cleavage/blastocyst media, as supports for human embryo culture.
Design: Prospective randomized study.
Setting: University-based IVF clinic.
Patient(s): Two hundred forty-nine patients undergoing IVF treatment for the first or second time, randomly allocated at the time of oocyte retrieval, to either culture in G1.2/G2.2 or Sydney IVF media.
Intervention(s): Oocyte recovery, IVF or intracytoplasmic sperm injection, embryo culture, transfer on day 3 or day 5/6.
Main Outcome Measure(s): Developmental stage on day 3, blastocyst rate, pregnancy outcome as assessed by βhCG positive test, implantation rates, and ongoing pregnancies.
Result(s): Embryos cultured in G1.2/G2.2 media displayed a faster kinetics of cleavage, compaction, blastulation, and hatching, but a lower day 3 embryo quality than those grown in Sydney IVF media. For patients with at least five embryos, G1.2/G2.2 media yielded higher implantation rates (26.2%) in our day 3 embryo transfer program when compared to Sydney IVF medium (15.5%), whereas similar implantation rates were obtained for day 5/6 embryo transfer for both media (43.1% and 36.1%, respectively).
Conclusion(s): In our day 3 embryo transfer program, G1.2/G2.2 media were superior to Sydney IVF media, whereas both media yielded similar outcomes in our blastocyst transfer program.
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Van Langendonckt, Anne
Demylle, Dominique
Wyns, Christine
NISOLLE, Michelle ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Donnez, Jacques
Language :
English
Title :
Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study.
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