Reference : Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the c...
Scientific journals : Article
Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)
Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study.
Van Langendonckt, Anne [ > > ]
Demylle, Dominique [ > > ]
Wyns, Christine [ > > ]
NISOLLE, Michelle mailto [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]
Donnez, Jacques [ > > ]
Fertility and Sterility
Yes (verified by ORBi)
New York
[en] Human embryo culture ; sequential culture medium ; IVF ; blastocyst ; implantation rate
[en] Objective: To compare two commercially available sequential media, G1.2/G2.2 and Sydney IVF cleavage/blastocyst media, as supports for human embryo culture.

Design: Prospective randomized study.

Setting: University-based IVF clinic.

Patient(s): Two hundred forty-nine patients undergoing IVF treatment for the first or second time, randomly allocated at the time of oocyte retrieval, to either culture in G1.2/G2.2 or Sydney IVF media.

Intervention(s): Oocyte recovery, IVF or intracytoplasmic sperm injection, embryo culture, transfer on day 3 or day 5/6.

Main Outcome Measure(s): Developmental stage on day 3, blastocyst rate, pregnancy outcome as assessed by βhCG positive test, implantation rates, and ongoing pregnancies.

Result(s): Embryos cultured in G1.2/G2.2 media displayed a faster kinetics of cleavage, compaction, blastulation, and hatching, but a lower day 3 embryo quality than those grown in Sydney IVF media. For patients with at least five embryos, G1.2/G2.2 media yielded higher implantation rates (26.2%) in our day 3 embryo transfer program when compared to Sydney IVF medium (15.5%), whereas similar implantation rates were obtained for day 5/6 embryo transfer for both media (43.1% and 36.1%, respectively).

Conclusion(s): In our day 3 embryo transfer program, G1.2/G2.2 media were superior to Sydney IVF media, whereas both media yielded similar outcomes in our blastocyst transfer program.

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