[en] Myeloperoxidase (MPO) is a protein of interest due to its involvement in equine pathologies. Until now, results in equine diagnostic research were achieved through extracellular MPO detection. However, studying the cellular MPO content in neutrophils has revealed important insights in human diseases. This study aimed to develop a technique for the specific detection of MPO on the single cell level defining a flow cytometric protocol for the detection of both equine surface-bound and cellular MPO. Both indirect and direct labeling techniques are described which include the comparison of two secondary antibodies and two linking-fluorochromes, respectively.
Research Center/Unit :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Wauters, Jella; Faculty of Veterinary Medicine, Ghent University > Pharmacology, Toxicology and Biochemistry
Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Pille, Frederik
Martens, Ann
Demeyere, Kristel
Sys, Stanislas
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Gasthuys, Frank
Meyer, Evelyne; Faculty of Veterinary Medicine, Ghent University > Pharmacology, Toxicology and Biochemistry
Language :
English
Title :
Flow cytometric detection of myeloperoxidase in horse neutrophils: a novel technique in equine diagnostic research.
Publication date :
2011
Journal title :
Veterinary Immunology and Immunopathology
ISSN :
0165-2427
eISSN :
1873-2534
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
144
Issue :
3-4
Pages :
417-22
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
IWT - Agency for Innovation by Science and Technology
Commentary :
Copyright (c) 2011 Elsevier B.V. All rights reserved.
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