Reference : IGF-II in primary human colorectal tumors: peptide level, activated promoters, parent...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
IGF-II in primary human colorectal tumors: peptide level, activated promoters, parental imprinting and gene rearrangement.
Winkler, Rose mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Delacroix, Laurence mailto [Université de Liège - ULiège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Bensbaho, K. [> > > >]
Lambert, Stéphanie [Centre Hospitalier Universitaire de Liège - CHU > > Salle Cardiologie NDB >]
Collette, Julien [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
Hodzic, D. [> > > >]
Hormone & Metabolic Research
Georg Thieme Verlag Stuttgart
Yes (verified by ORBi)
[en] Adenocarcinoma/chemistry/genetics ; Colonic Neoplasms/chemistry/genetics ; DNA Primers ; DNA, Neoplasm/analysis ; Gene Expression Regulation, Neoplastic ; Gene Rearrangement ; Genomic Imprinting ; Humans ; Insulin-Like Growth Factor II/analysis/genetics ; Polymorphism, Restriction Fragment Length ; Promoter Regions, Genetic/physiology ; RNA, Messenger/analysis ; RNA, Neoplasm/analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor Cells, Cultured
[en] IGF-II is a polypeptide growth factor with growth and differentiation promoting activities, involved in human development. We have reported previously IGF-II mRNA and peptide overexpression in primary human colon cancers. Here we show that the IGF-II peptide content is increased in six primary colon cancers compared to the corresponding healthy tissues. The IGF-II transcripts in healthy and cancerous colon tissues were identified by Northern blotting and RT-PCR. Promoters P3 and P4 were active in most tissues. Relaxation of parental imprinting was observed in two tumors and one healthy tissue, without any correlation with the IGF-II transcript levels. Rearrangements of the IGF-II gene in two tumors containing very high amounts of IGF-II mRNA are described. Fragments containing the breakpoints were cloned by the vectorette-PCR strategy. In both tumors, the breakpoints occurred in repetitive sequences. In one tumor (T11), the breakpoint was localized 2 kb downstream the end of exon 9. The second tumor (T18) contains two modified alleles. In one rearranged allele the breakpoint is located in exon 9. The exact position of the breakpoint in the second rearranged allele has not been identified. In future experiments, the correlation between the gene rearrangements and IGF-II mRNA overexpression will be studied.
Université de Liège
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS

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