[en] OBJECTIVE: To use Doppler ultrasonography and single-fiber laser Doppler flowmetry (LDF) to evaluate blood flow in the dependent and nondependent hind limbs of anesthetized horses and to evaluate changes in femoral arterial blood flow and microvascular skeletal muscle perfusion in response to administration of phenylephrine hydrochloride or dobutamine hydrochloride. ANIMALS: 6 healthy adult horses. PROCEDURE: Horses were anesthetized and positioned in left lateral recumbency. Doppler ultrasonography was used to measure velocity and volumetric flow in the femoral vessels. Single-fiber LDF was used to measure relative microvascular perfusion at a single site in the semimembranosus muscles. Phenylephrine or dobutamine was then administered to decrease or increase femoral arterial blood flow, and changes in blood flow and microvascular perfusion were recorded. RESULTS: Administration of phenylephrine resulted in significant decreases in femoral arterial and venous blood flows and cardiac output and significant increases in mean aortic blood pressure, systemic vascular resistance, and PCV. Administration of dobutamine resulted in significant increases in femoral arterial blood flow, mean aortic blood pressure, and PCV. Significant changes in microvascular perfusion were not detected. CONCLUSION AND CLINICAL RELEVANCE: Results suggest that Doppler ultrasonography and single-fiber LDF can be used to study blood flows in the hind limbs of anesthetized horses. However, further studies are required to determine why changes in femoral arterial blood flows were not associated with changes in microvascular perfusion
Disciplines :
Veterinary medicine & animal health Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Raisis, Anthea L
Young, Lesley E
Taylor, Polly M
Walsh, Karen P
Lekeux, Pierre ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie - Doyen de la Faculté de Médecine vétérinaire
Language :
English
Title :
Doppler ultrasonography and single-fiber laser Doppler flowmetry for measurement of hind limb blood flow in anesthetized horses
Publication date :
2000
Journal title :
American Journal of Veterinary Research
ISSN :
0002-9645
eISSN :
1943-5681
Publisher :
American Veterinary Medical Association, Schaumburg, United States - Illinois
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Bibliography
Raisis AL, Young LE, Meire HB, et al. Measurement of hindlimb blood flow recorded using Doppler ultrasound during administration of vasoactive agents in halothane-anesthetized horses. Vet Radiol Ultrasound 2000;in press
Evans DH, McDicken WN, Skidmore R, et al. Doppler ultrasound. Physics, instrumentation and clinical application. Chichester, UK: John Wiley & Sons, 1989;162-187.
Norman WM, Court MH, Dodman NH, et al. Measurement of muscle surface capillary blood flow by laser Doppler flowmetry. Vet Surg 1992;21:491-493.
Serteyn D, Coppens P, Mottart E, et al. Measurements of muscular microcirculation by laser Doppler flowmetry in isoflurane and halothane anesthetised horses. Vet Rec 1987;121:324-326.
Serteyn D, Lavergne L, Coppens P, et al. Equine postanaesthetic myositis: muscular post ischaemic hyperaemia measured by laser Doppler flowmetry. Vet Rec 1988;123:126-128.
Micheels J, Alsbjorn B, Sorensen B. Laser Doppler flowmetry. A new non-invasive measurement of microcirculation in intensive care? Resuscitation 1984;12:31-39.
Oberg PA. Laser Doppler flowmetry. Crit Rev Biomed Eng 1990;18:125-163.
Stern MD. In vivo evaluation of microcirculation by coherent light scattering. Nature 1975;254:56-58.
Nilsson G. Laser Doppler flowmeter. IEEE Transactions on Biomedical Engineering 1980;BME-27:597-604.
Smits GJ, Roman RJ, Lombard JH. Evaluation of laser-Doppler flowmetry as a measure of tissue blood flow. J Appl Physiol 1986;61:666-672.
Tymyl K, Roman RJ, Lombard JH. Blood flow in skeletal muscle. Dev Cardiovasc Med. 1990;107:215-226.
Serteyn D, Mottart E, Michaux C, et al. Laser Doppler flowmetry: muscular microcirculation in anaesthetised horses. Equine Vet J 1986;18:391-395.
Hennig GE. The effect of xylazine on equine muscle surface capillary blood flow, J Vet Pharmacol Therap 1995;18:388-390.
Kvernebo K, Staxrud LE, Salerud EG. Assessment of human muscle perfusion with single fibre laser Doppler flowmetry. Microvascular Research 1990;39:376-385.
Larsson SE, Cai H, Zhang Q, et al. Measurement by laser Doppler flowmetry of microcirculation in lower leg muscle at different blood fluxes in relation to electromyographically determined contraction and accumulated fatigue. Eur J Appl Physiol 1995;70:288-293.
Salerud EG, Oberg PA. Single-fibre laser Doppler flowmetry: a method for deep tissue perfusion measurements. Med Biol Eng Comput 1987;25:329-334.
Steffey EP, Howland D, Giri S, et al. Enflurane, halothane, and isoflurane potency in horses. Am J Vet Res 1977;38:1037-1039.
Grossman W. Cardiac catheterisation. In: Braunwald E, ed. Heart disease: a textbook of cardiovascular medicine. Philadelphia: WB Saunders Co, 1991;182-183.
Young LE, Blissitt KJ, Bartram DH, et al. Measurement of cardiac output by transoesophageal Doppler echocardiography in anaesthetised horses: comparison with thermodilution. Br J Anaesth 1996;77:773-780.
Nichols WW, O'Rourke ME The nature of flow of a liquid. In: Nichols WW, O'Rourke ME eds. McDonald's blood flow in arteries: theoretical, experimental and clinical principles. London: Arnold, 1998;11-53.
Lee Y-HL, Clarke KW, Alibhai HIK, et al. Effects of dopamine, dobutamine, dopexamine, phenylephrine, and saline solution on intramuscular blood flow and other cardiopulmonary variables in halothane-anesthetized ponies. Am J Vet Res 1998;59:1463-1472.
Lindsay WA, Pascoe PJ, McDonell WN, et al. Effect of protective padding on forclimb intracompartmental muscle pressures in anesthetized horses. Am J Vet Res, 1985;46:688-691.
White NA, Saurez M. Change in triceps muscle intracompartmcntal pressure with repositioning and padding of the lower-most thoracic limb of the horse. Am J Vet Res 1986;47:2257-2260.
Weaver BMQ, Lunn CEM, Staddon GE. Muscle perfusion in the horse. Equine Vet J 1984;16:66-68.
Matsen FA, Krugmire RB, King RV. Increased tissue pressure and its effect of muscle oxygenation in level and elevated human limbs. Clin Orthop Rel Res 1979;144:311-320.
Tangelder GJ, Slaaf DW, Reneman RS. Skeletal muscle microcirculation and changes in transmural and perfusion pressure. Prog Appl Microcirc 1984;5:93-108.
Smith JJ, Kampine JP. The peripheral circulation and its regulation. In: Satterfield TS, ed. Circulatory physiology - the essentials. Baltimore: The Williams & Wikins Co, 1989;140-160.
Lindbom L, Arfos K-E. Interrelationship between arteriolar blood flow distribution and capillary perfusion in skeletal muscle. Prog Appl Microcirc 1984;5:84-92.
Lewis DH. The response of the microvasculature in skeletal muscle to haemorrhage, trauma and ischaemia. Prog Appl Microcirc 1984;5:127-138.
Wheatley AM, Hickman R. The influence of flow and hematocrit on the laser Doppler flux signal from the surface of the perfused pig liver. Microcirculation 1995;2:19-25.
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