Reference : Retinoic acid induces TGFbeta-dependent autocrine fibroblast growth.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Retinoic acid induces TGFbeta-dependent autocrine fibroblast growth.
Fadloun, A. [> > > >]
Kobi, D. [> > > >]
Delacroix, Laurence mailto [Université de Liège - ULiège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Dembele, D. [> > > >]
Michel, I. [> > > >]
Lardenois, A. [> > > >]
Tisserand, J. [> > > >]
Losson, R. [> > > >]
Mengus, G. [> > > >]
Davidson, I. [> > > >]
Nature Publishing Group
Yes (verified by ORBi)
United Kingdom
[en] Animals ; Autocrine Communication/drug effects/genetics ; Base Sequence ; COS Cells ; Cell Proliferation/drug effects ; Cells, Cultured ; Cercopithecus aethiops ; Connective Tissue Growth Factor ; Consensus Sequence ; Fibroblasts/drug effects/physiology ; Gene Expression Profiling ; Gene Expression Regulation/drug effects ; Immediate-Early Proteins/genetics ; Intercellular Signaling Peptides and Proteins/genetics ; Mice ; Receptor Cross-Talk/drug effects ; Receptors, Retinoic Acid/metabolism ; Response Elements ; Transcription Factor TFIID/genetics/metabolism/physiology ; Transforming Growth Factor beta/genetics/physiology ; Tretinoin/pharmacology
[en] To evaluate the role of murine TFIID subunit TAF4 in activation of cellular genes by all-trans retinoic acid (T-RA), we have characterized the T-RA response of taf4(lox/-) and taf4(-/-) embryonic fibroblasts. T-RA regulates almost 1000 genes in taf4(lox/-) cells, but less than 300 in taf4(-/-) cells showing that TAF4 is required for T-RA regulation of most, but not all cellular genes. We further show that T-RA-treated taf4(lox/-) cells exhibit transforming growth factor (TGF)beta-dependent autocrine growth and identify a set of genes regulated by loss of TAF4 and by T-RA corresponding to key mediators of the TGFbeta signalling pathway. T-RA rapidly and potently induces expression of connective tissue growth factor (CTGF) via a conserved DR2 type response element in its proximal promoter leading to serum-free autocrine growth. These results highlight the role of TAF4 as a cofactor in the cellular response to T-RA and identify the genetic programme of a novel cross talk between the T-RA and TGFbeta pathways that leads to deregulated cell growth.
Giga-Neurosciences ; IGBMC

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