Faculty of 1000 Biology: evaluations for Colson S et al Microbiology 2008 Feb 154 (Pt 2) :373-82 http://www.f1000biology.com/article/id/1104344/evaluation. This paper dissects some aspects of the sensing and uptake of chitin and its degradation products as key elements in the decision of streptomycetes to produce reproductive aerial mycelium. This is an exciting glimpse into the significance of the likely co-evolution of streptomycetes with their mycelial fungal eukaryotic counterparts in the early rhizosphere, with fungal cell walls possibly being the/a major nutrient source at the time when the complex development of streptomycetes evolved. The future linking of these first steps in signal transduction with the well-known core developmental genes can be anticipated.
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[en] Streptomycetes are mycelial soil bacteria that undergo a developmental programme that leads to sporulating aerial hyphae. As soil-dwelling bacteria, streptomycetes rely primarily on natural polymers such as cellulose, xylan and chitin for the colonization of their environmental niche and therefore these polysaccharides may play a critical role in monitoring the global nutritional status of the environment. In this work we analysed the role of DasA, the sugar-binding component of the chitobiose ATP-binding cassette transport system, in informing the cell of environmental conditions, and its role in the onset of development and in ensuring correct sporulation. The chromosomal interruption of dasA resulted in a carbon-source-dependent vegetative arrest phenotype, and we identified a second DasR-dependent sugar transporter, in addition to the N-acetylglucosamine phosphotransferase system (PTS(GlcNAc)), that relates primary metabolism to development. Under conditions that allowed sporulation, highly aberrant spores with many prematurely produced germ tubes were observed. While GlcNAc locks streptomycetes in the vegetative state, a high extracellular concentration of the GlcNAc polymer chitin has no effect on development. The striking distinction is due to a difference in the transporters responsible for the import of GlcNAc, which enters via the PTS, and of chitin, which enters as the hydrolytic product chitobiose (GlcNAc(2)) through the DasABC transporter. A model explaining the role of these two essentially different transport systems in the control of development is provided.
Disciplines :
Microbiology
Author, co-author :
Colson, Séverine ; Université de Liège - ULiège > Centre d'ingénierie des protéines
van Wezel, G. P.
Craig, Matthias ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Noens, E. E.
Nothaft, H.
Mommaas, A. M.
Titgemeyer, F.
Joris, Bernard ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Rigali, Sébastien ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Language :
English
Title :
The Chitobiose-Binding Protein, DasA, Acts as a Link between Chitin Utilization and Morphogenesis in Streptomyces Coelicolor
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