33 	Amplified rDNA restriction analysis and further genotypic characterisation of metal-resistant soil bacteria and related facultative hydrogenotrophs

Brim, Hassan; Heyndrickx, Marc; De Vos, Paul; Wilmotte, Annick; Springael, Dirk; Schlegel, H. G.; Mergeay, Max

doi:10.1016/S0723-2020(99)80073-3

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33 Amplified rDNA restriction analysis and further genotypic characterisation of metal-resistant soil bacteria and related facultative hydrogenotrophs

Brim, Hassan; Heyndrickx, Marc; De Vos, Paul et al.

1999 • In Systematic and Applied Microbiology, 22, p. 258-268

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https://hdl.handle.net/2268/95928

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10.1016/S0723-2020(99)80073-3

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10390877

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Keywords :

ARDRA; 16S rRNA; Ralstonia; Molecular taxonomy; metal resistance

Abstract :

[en] The level of genotypic relationship between czc+ soil bacteria mainly resistant to zinc (but also to various other metals), and related facultative hydrogenotrophs previously assigned to the genera Alcaligenes, Ralstonia, and Burkholderia was evaluated using ARDRA (Amplified Ribosomal DNA Restriction Analysis). The analysis included 44 strains isolated from harsh industrial environments in sediments, soils and wastes with high content of heavy metals. These strains were selected by their ability to grow in the presence of high concentrations of multiple heavy metals and to hybridise with czc or ncc probes. The czc operon confers resistance to cadmium, zinc and cobalt in strain Ralstonia eutropha CH34. The net operon confers resistance to nickel, cobalt and cadmium in strain 31A known as Alcaligenes xylosoxidans. The analysis showed a close phylogenetic clustering of the czc+ strains inside the Ralstonia genus despite of their different origins and that the Ralstonia genus contained also the hydrogenotrophs and some catabolic strains assigned to the genus Ralstonia eutropha, strains up to now registrated as CDC IV c-2 strains as well as reference strains belonging to Ralstonia solanacearum and Ralstonia pickettii. The ncc+ strains are phylogenetically less related to each other compared to the czc+ strains. This suggests that the tested czc+ strains and some of the ncc+ strains may be considered as belonging to the genus Ralstonia. Inside this major Ralstonia cluster, a subcluster gathers most of the czc+ isolates maybe giving a clue to define a new species. Besides, from 30 tested strains, 15 metal resistant strains of this subcluster proved to display the unusual mutator phenotype characteristic of the representative strain CH34

Research center :

Flemish Institute of Technology (VITO)

Disciplines :

Microbiology

Author, co-author :

Brim, Hassan

Heyndrickx, Marc

De Vos, Paul

Wilmotte, Annick ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes

Springael, Dirk

Schlegel, H. G.

Mergeay, Max ; Université de Liège - ULiège > Département Argenco : Secteur GeMMe > Génie minéral et recyclage

Language :

English

Title :

33 Amplified rDNA restriction analysis and further genotypic characterisation of metal-resistant soil bacteria and related facultative hydrogenotrophs

Publication date :

1999

Journal title :

Systematic and Applied Microbiology

ISSN :

0723-2020

eISSN :

1618-0984

Publisher :

Elsevier GmbH, Urban & Fischer Verlag, Jena, Germany

Volume :

Pages :

258-268

Peer reviewed :

Peer Reviewed verified by ORBi

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http://www.scopus.com/record/display.url?eid=2-s2.0-0032999894&origin=resultslist&sort=plf-f&src=s&nlo=&nlr=&nls=&imp=t&sid=mK1Xkb8Buelw_W8_Cig8GlW%3a210&sot=br&sdt=a&sl=56&s=SOURCE-ID%2820861%29+AND+PUBYEAR+IS+1999+AND+NOT+DOCTYPE%28ip%29&relpos=52&relpos=12&searchTerm=SOURCE-ID%2820861%29%20AND%20PUBYEAR%20IS%201999%20AND%20NOT%20DOCTYPE%28ip%29

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