Reference : 'Soil microbial diversity and forest ecosystem functioning
Scientific congresses and symposiums : Unpublished conference/Abstract
Life sciences : Environmental sciences & ecology
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/94566
'Soil microbial diversity and forest ecosystem functioning
English
Carnol, Monique mailto [Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Ecologie végétale et microbienne >]
Apr-2004
No
No
National
Biodiversity: state, stakes and future
du 7 avril au 9 avril 2004
Louvain-La-Neuve
Belgique
[en] nitrification ; ammonia-oxidizing-bacteria ; PCR-DGGE ; liming ; elevated CO2
[en] The term ‘biodiversity’ has been defined as ‘The variety of life in all its forms, levels and combinations’, with the concept including several scales (ecosystem diversity, species diversity, and genetic diversity) and processes related to ecosystem functioning (role in food webs, primary productivity, biogeochemical cycling). Ecosystem diversity comprises plant, animal and microbial diversity. Whereas much research focuses on plant and animal diversity, complexity of interactions and methodological difficulties have so far limited research on microbial diversity. However, within the soils of forest ecosystems, microorganisms are responsible for key functions such as organic matter decomposition and mineralisation, in particular within the C and N cycles. These processes are closely linked to nutrient availability and therefore play an important role in stand productivity, tree health and ecosystem functioning. Within the attempts to use soil microbial communities as indicators of soil health, microorganisms and their functions have been classified according to their sensitivity to perturbations. Processes of mineralization, linked to soil productivity, were ranked with the highest priority. Because of the small number of organisms involved and their key role in nutrient cycling, nitrifying bacteria and the nitrification process were identified as very sensitive to environmental perturbation.
In this paper, I present an overview of ongoing studies investigating the link between the nitrification process and the diversity of ammonia-oxidisers, bacteria responsible for the first, rate-limiting step of the nitrification process. Ammonia-oxidiser community structure was investigated using a PCR-based approach targeting the 16S rRNA gene of beta-subgroup ammonia oxidisers, followed by DGGE (Denaturing Gradient Gel Electrophoresis) and sequence analysis. The analysis of community structure was combined with more traditional measurements of nitrate production and soil characteristics. Investigations included several Belgian forest ecosystems and the effects of environmental factors, such as liming and the effects of a 4-year exposure to elevated CO2.
Researchers
http://hdl.handle.net/2268/94566

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