Reference : Le clonage par transfert de noyau dans l'espece bovine: resultats et perspectives.
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Le clonage par transfert de noyau dans l'espece bovine: resultats et perspectives.
[en] Cloning using nuclear transfer in bovine species: results and perspectives
Ectors, Fabien mailto [Université de Liège - ULiège > > GIGA-Management : Plate-forme transgénique >]
Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique
Académie Royale de Médecine de Belgique
[en] Animals ; Birth Weight ; Cattle/physiology ; Cloning, Organism/methods/veterinary ; Embryo Transfer/methods/veterinary ; Female ; Fertilization in Vitro/veterinary ; Male ; Pregnancy ; Pregnancy, Prolonged
[en] A all in vitro cloning technique was developed in which the reconstituted embryos from the first cycle nuclear transfer (cloning) were used as blastomere donor for the second cycle nuclear transfer (recloning). Such method permitted to produce 14.5% of morulae and 14.9% of blastocysts after the first and second cycles of nuclear transfer, respectively. The rates of birth obtained after transfer of such embryos were 21.4% et 20.8% for first and second cycles respectively, corresponding to 6 et 5 calves for 28 et 24 transferred embryos. Unfortunately, gestation pathologies and an increase of birth weights were observed. It seems that the in vitro presence of gametes and/or embryos may be responsible of an alteration in the control of gene expression.
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