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Nuclear transplantation using bovine primordial germ cells from male fetuses.
Delhaise, F.; Ectors, Fabien; de Roover, R. et al.
1995In Reproduction, Fertility and Development, 7 (5), p. 1217-9
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Keywords :
Animals; Blastocyst/physiology; Cattle/embryology; Culture Techniques; Embryo Transfer; Female; Fetus/ultrastructure; Male; Morula/physiology; Nuclear Transfer Techniques; Oocytes/ultrastructure; Pregnancy; Spermatozoa/physiology/ultrastructure
Abstract :
[en] The developmental potential of nuclei of bovine gonial cells was investigated by nuclear transfer. Gonial cells were collected from male fetuses at about 175 days post coitum (p.c.). They were fused with enucleated oocytes; reconstituted embryos were cultured in vitro for 7 days. Embryos reaching the compacted morula or blastocyst stage were either fixed for cell counting or transferred into recipients. Out of 115 oocyte-gonia fusions, 101 (87.8%) gave rise to cleaved embryos at Day 3 and 26 (22.6%) had reached the 8-cell stage. At Day 7, 1 (1%) developed to the morula stage and 5 (4%) reached the blastocyst stage. Three blastocysts were fixed and showed normal cell numbers (135; 90; 76 cells). Three blastocysts and one morula were transferred in four recipients; two recipients were pregnant at Day 21 but only one was positive at Day 35 p.c.; this last one aborted around Day 40 p.c. No conceptus was collected. These results indicate that gonial cell nuclei can be partially reprogrammed; they are able to develop into blastocysts and to initiate gestation. However, more experiments will be necessary to prove the nuclear totipotency of bovine gonial cells.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Delhaise, F.
Ectors, Fabien  ;  Université de Liège - ULiège > GIGA-Management : Plate-forme transgénique
de Roover, R.
Ectors, Francis
Dessy, F.
Language :
English
Title :
Nuclear transplantation using bovine primordial germ cells from male fetuses.
Publication date :
1995
Journal title :
Reproduction, Fertility and Development
ISSN :
1031-3613
eISSN :
1448-5990
Publisher :
CSIRO Publishing, East Melbourne, Australia
Volume :
7
Issue :
5
Pages :
1217-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 14 April 2011

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