[en] The distribution of fibronectin and the morphological differentiation of the genital ducts was studied in rat fetuses at ages from 15 to 21 days. Fibronectin was localized with the peroxidase-antiperoxidase and avidin-biotin method at the electron- and light-microscope level. In 15-day-old male and female fetuses, fibronectin was localized as a continuous lamella around the mesonephric duct and as a discontinuous lamella around the paramesonephric duct. During the differentiation of the female paramesonephric duct, the fibronectin layer became continuous and remained so after the age of 16 days. The fibronectin layer of the male mesonephric duct remained continuous at all ages. The accumulation of mesenchymal cells on the outer surface of the female mesonephric duct and the concomitant detachment of the fibronectin layer around the duct suggests that mesenchymal regulation plays a role in the regression of the mesonephric duct. In the regressing male paramesonephric duct fibronectin was simultaneously lost in the condensed periductal mesenchyme, the places of epithelio-mesenchymal contact, and the epithelial cytoplasmic protrusions towards the mesenchyme. Ultrastructurally, fibronectin was localized in the basal laminae, on the cell membrane in contact with the extracellular material, and on the surface of the fibrillar and flocculent extracellular material. In addition to auto- and heterophagy, epithelio-mesenchymal interactions seem to play an important role in the regression of the genital ducts, although in different ways in males and females. The present results give additional support to the theory of the possible migration of epithelial cells into the surrounding mesenchyme during the regression of the paramesonephric duct.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Paranko, J.
Pelliniemi, L. J.
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Language :
English
Title :
Epithelio-mesenchymal interface and fibronectin in the differentiation of the rat mesonephric and paramesonephric ducts.
Chemnitz J, Christensen BC (1983) Intracellular and extracellular localization of fibronectin in primary cultures of aortic smooth muscle cells using immunoperoxidase cytochemistry in electron microscopy. Eur J Cell Biol 30:200-204.
Courtoy PJ, Boyles J (1983) Fibronectin in the microvasculaure: localization in the pericyte-endothelial interstitium. J Ultrastruct Res 83:258-273.
Courtoy PJ, Picton DH, Farquhar MG (1983) Resolution and limitations of the immunoperoxidase procedure in the localization of extracellular matrix antigens. J Histochem Cytochem 31:945-951.
Cunha GR, Shannon JM, Neubauer BL, Sawyer LM, Fujii H, Taguchi O, Chung LWK (1981) Mesenchymal-epithelial interactions in sex differentiation. Hum Genet 58:68-77.
Donahoe PK, Budzik GP, Trelstad R, Mudgett-Hunter M, Fuller A, Hutson JM, Ikawa H, Hayashi A, MacLaughlin D (1982) Müllenan-inhibiting substance: an update. Recent Prog Horm Res 38:279-330.
Dyche WJ (1979) A comparative study of the differentiation and involution of the Müllerian duct and Wolffian duct in the male and female fetal mouse. J Morphol 162:175-210.
Ekblom P (1981) Determination and differentiation of the nephron. Med Biol 59:139-160.
Engvall E, Ruoslahti E, Miller EJ (1978) Affinity of fibronectin to collagens of different genetic types and to fibrinogen. J Exp Med 147:1584-1595.
Flickinger CJ (1969) Fine structure of the Wolftian duct and cytodifferentiation of the epididymis in fetal rats. Z Zellforsch Mikrosk Anat 96:344-360.
Furcht LT, Smith D, Wendelschafer-Crabb G, Mosher DF, Foidart JM (1980) Fibronectin presence in native collagen fibrils of human fibroblasts: immunoperoxidase and Immun ferritin localization. J Histochem Cytochem 28:1319-1333.
Hascall VC, Hascall GK (1981) Proteoglycans. Cell biology of extracellular matrix, ch. 2 , ED Hay, Plenum Press, New York; 39-63.
Hayashi A, Donahoe PK, Budzik GP, Trelstad RL (1982) Peri ductal and matrix glycosaminoglycans in rat Müllerian duct development and regression. Dev Biol 92:16-26.
Hedman K, Vaheri A, Wartiovaara J (1978) External fibronectin of cultured human fibroblasts is predominantly a matrix protein. J Cell Biol 76:748-760.
Hedman K, Johansson S, Vartio T, Kjellh L, Vaheri A, Höö M (1982) Structure of the pericellular matrix: association of heparan and chondroitin sulfates with fibronectin-procollagen fibers. Cell 28:663-671.
Hølund B, Clausen PP, Clemmensen I (1981) The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue. Histochemistry 72:291-299.
Hynes RO (1981) Fibronectin and its relation to cellular structure and behavior. Cell biology of extracellular matrix, ch. 10 , ED Hay, Plenum Press, New York; 295-333.
Josso N, Picard J-Y, Tran D (1977) The anti-Mullerian hormone. Recent Prog Horm Res 33:117-167.
.
Kellokumpu-Lehtinen P, Santti RS, Pelliniemi LJ (1981) Development of human fetal prostate in culture. Urol Res 9:89-98.
Kleinman HK, Klebe RJ, Martin GR (1981) Role of collagenous matrices in the adhesion and growth of cells. J Cell Biol 88:473-485.
Laurie GW, Leblond CP, Martin GR (1983) Light-microscopic immunolocalization of type IV collagen, laminin, heparan sul-fate proteoglycan, and fibronectin in the basement membranes of a variety of rat organs. Am J Anat 167:71-82.
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077-1083.
Martinez-Hernandez A, Amenta PS (1983) The basement membrane in pathology. Lab Invest 48:656-677.
Mayer BW, Hay ED, Hynes RO (1981) Immunocytochemical localization of fibronectin in embryonic chick trunk and area vasculosa. Dev Biol 82:267-286.
Monaghan P, Warburton MJ, Perusinghe N, Rudland PS (1983) Topographical arrangemcnt of basement membrane proteins in lactating rat mammary gland: comparison of the distribution of type IV collagen, laminin, fibronectin, and Thy-I at the ultrastructural level. Proc Natl Acad Sci USA 80:3344-3348.
Mudgett-Hunter M, Budzik GP, Sullivan M, Donahoe PK (1982) Monoclonal antibody to Mullerian inhibiting substance. J Immunol 128:1327-1333.
Paranko J, Pelliniemi LJ, Vaheri A, Foidart J-M, Lakkala-Paranko T (1983) Morphogenesis and fibronectin in sexual differentiation of rat embryonic gonads. Differentiation 23:72-81.
Pelliniemi LJ, Dym M, Karnovsky MJ (1980) Peroxidase histochemistry using diaminobenzidine tetra hydrochloride stored as a frozen solution. J Histochem Cytochem 28:191-192.
Pelliniemi LJ, Kellokumpu-Lehtinen P, Tapanainen J, Hoffer AP, Huhtaniemi I (1981) Ultrastructural and hormonal differentiation of the human prenatal testis and the mesonephric duct. Development and function of reproductive organs , AG Byskov, H Peters, International Congress Series no. 559. Proceedings of the Vth workshop on the development and function of the reproductive organs. Excerpta Medica, Amsterdam; 61-65.
Price JM, Donahoe PK, Ito Y, Hendren WH (1977) Programmed cell death in the Mullerian duct induced by Mullerian inhibiting substance. Am J Anat 149:353-376.
Ruoslahti E, Engvall E, Hayman EG (1981) Fibronectin: current concepts of its structure and functions. Coll Relat Respondent 1:950-968.
Sanders EJ (1982) Ultrastructural immunocytochemical localization of fibronectin in the early chick embryo. J Embryol Exp Morphol 71:155-170.
Stenman S, Vaheri A (1978) Distribution of a major connective tissue protein, fibronectin, in normal human tissues. J Exp Med 147:1054-1064.
Sternberger LA Immunocytochemistry, 2nd edn., John Wiley & Sons, New York; 1979.
Stinnakre M-G (1975) Période de sensibilité aux androgens du canal de Wolff de foetus de rat. Arch Anat Microsc Morphol Exp 64:45-59.
Thesleff I, Barrach HJ, Foidart JM, Vaheri A, Pratt RM, Martin GR (1981) Changes in the distribution of type IV collagen, laminin, proteoglycan, and fibronectin during mouse tooth development. Dev Biol 81:182-192.
Tran D, Josso N (1982) Localization of anti-Mullerian hormone in the rough endoplasmic reticulum of the developing bovine Sertoli cell using immunocytochemistry with a monoclonal antibody. Endocrinology 111:1562-1567.
Trelstad RL, Hayashi A, Hayashi K, Donahoe PK (1982) The epithelial-mesenchymal interface of the male rat Mullerian duct: loss of basement membrane integrity and ductal regression. Dev Biol 92:27-40.
Warburton MJ, Ferns SA, Rudland PS (1982) Enhanced synthesis of basement membrane proteins during the differentiation of rat mammary tumour epithelial cells into myoepithelial- like cells in vitro. Exp Cell Res 137:373-380.
Wartiovaara J, Leivo I, Vaheri A (1979) Expression of the cell surface-associated glycoprotein, fibronectin, in the early mouse embryo. Dev Biol 69:247-257.
Vigier B, Picard J-Y, Josso N (1981) A hybridoma against anti-Müllerian hormone. Development and function of reproductive organs. International Congress series No 559. Proceedings of the Vth workshop on the development and function of the reproductive organs , AG Byskov, H Peters, Excerpta Medica, Amsterdam; 66-72.
Vigier B, Picard J-Y, Bézard J, Josso N (1981) Anti-Mullerian hormone: A local or long-distance morphogenetic factor?. Hum Genet 58:85-90.
Yamada KM (1981) Fibronectin and other structural proteins. Cell biology of extracellular matrix, ch. 4 , ED Hay, Plenum Press, New York; 95-114.
