mass spectrometry; DNA; spectroscopy; electronic; electron; photodetachment; synchrotron; photodissociation; MS/MS
Abstract :
[en] DNA polyanions trapped in a mass spectrometer undergo electron detachment following UV irradiation. Photodetachment is a single-photon process. Its efficiency depends on the nature of the DNA bases, the ion's charge, and the excitation wavelength. Photodetachment can therefore be used to perform ion spectroscopy experiments, which probe electronic excitation within the initial charge state of the nucleic acids. Ion spectroscopy experiments on trapped nucleic acid cations and anions were performed from 4 to 20 eV using an OPO laser or using synchrotron radiation. Photoelectron spectroscopy experiments were also performed on multiply charged anions to probe direct detachment cross sections and electronic excitations within the final charge. The electronic spectra obtained from photodetachment integral cross sections show several resonances, provided that the photon energy is larger than the electron binding energy. We will also discuss whether the electronic spectra obtained via photodetachment can be used to probe gas phase ion structure.
Research Center/Unit :
Giga-Systems Biology and Chemical Biology - ULiège
Disciplines :
Chemistry Physics
Author, co-author :
Gabelica, Valérie ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Rosu, Frédéric ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Joly, Laure ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Matheis, Katerina; Karlsruher Institut für Technologie (KIT), Institute of Physical Chemistry, Karlsruhe, 76131, Germany
Kappes, Manfred M.; Karlsruher Institut für Technologie (KIT), Institute of Physical Chemistry, Karlsruhe, 76131, Germany
Giuliani, Alexandre; Synchrotron Soleil, Gif-sur-Yvette, 91192, France
Miloslavljevic, Alexandar; Synchrotron Soleil, Gif-sur-Yvette, 91192, France
Refregiers, Matthieu; Synchrotron Soleil, Gif-sur-Yvette, 91192, France
Nahon, Laurent; Synchrotron Soleil, Gif-sur-Yvette, 91192, France
Antoine, Rodolphe; University Lyon 1, Laboratory of Molecular and Ionic Spectroscopy (LASIM), Villeurbanne, 69622, France
Dugourd, Philippe; University Lyon 1, Laboratory of Molecular and Ionic Spectroscopy (LASIM), Villeurbanne, 69622, France
Language :
English
Title :
Electronic spectroscopy of nucleic acids in the gas phase
Publication date :
2010
Event name :
240th ACS National Meeting
Event organizer :
ACS, PHSY division, J. Matthias Weber & Jack Simons
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