Localization by immunofluorescent microscopy of several collagen types and of a basement membrane proteoglycan in rat glomerular epithelial and mesangial cell cultures.
Foidart, J. B.; Foidart, Jean-Michel; Hassell, J.et al.
[en] Confluent cultures of rat glomerular epithelial and mesangial cells were studied by immunofluorescent microscopy, using affinity-purified antibodies directed against collagen of type I-V or an antiserum directed against a basement membrane (BM) proteoglycan. The epithelial cells were stained by antibodies directed against type I, IV and V collagen, whereas the mesangial cells were stained by all the antibodies directed against the different collagenous antigens tested. Therefore, only mesangial cells contained antigenic determinants of type III collagen. On the contrary, both cell types possessed BM proteoglycan antigens. The data suggest that rat glomerular epithelial and mesangial cells may be implicated in the biosynthesis of different components of normal (and pathological) glomerular BM.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Foidart, J. B.
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Hassell, J.
Mahieu, P.
Language :
English
Title :
Localization by immunofluorescent microscopy of several collagen types and of a basement membrane proteoglycan in rat glomerular epithelial and mesangial cell cultures.
Burgeson R. E.; El Adli. A.F.; Kaitila. I.I.; Hallister. D. W.: Fetal membrane collagens. Identification of two new collagen alpha chains. Proc. natn. Acad. Sci. USA 73: 2579-2583 (1976).
Burke J.M.: Balian, G.; Ross. R.: Bornstein. P.: Synthesis of type I and III procollagen and collagen by monkey aortic smooth muscle cells in vitro. Biochemistry 16: 3243-3249 (1977).
Foidart, J. B.: Dechenne, C.A.; Mahieu, P. R.: Creutz, C. E.: De Mey, J.: Tissue culture of normal glomeruli. Isolation and morphological characterization of two homogeneous cell lines. Invest. Cell Pathol. 2: 15-28 (1979).
Foidart. J B.: Dechenne, C. A.: Dubois, C.: Deheneffe. J.: Mahieu, P.R.: Tissue culture of isolated renal glomeruli. Present and future. Adv. Nephrol. 10: 267-292 (1980).
Foidart. J.B.: Pirard. Y.S.: Winand. R.J.: Mahieu. P. R.: Tissue culture of normal rat glomeruli. Gly-cosaminoglycan biosynthesis by homogeneous epithelial and mesangial cell populations. Renal Physiol. Basel 3: 169-173 (1980).
Foidart, J. B.: Dechenne, C.A.: Mahieu, P. R.: Tissue culture of normal rat glomeruli. Characterization of collagenous and non-collagenous basement membrane antigens on the epithelial and mesangial cells. Diagnostic Histol. 4: 71-77 (1981).
Foidart. J. M.; Berman. J.J.: Paglia. L.; Rennard. S.; Abe, S.: Perantoni, A.; Martin. G. R.: Synthesis of fibronectin. laminin. and several collagens by a liver-derived epithelial line. Lab. Invest. 42:525-532 (1980).
Foidart. J. M.: Foidart. J. B.; Mahieu, P. R.: Synthesisof collagen and fibronectin by glomerular cells in culture. Renal Physiol. Basel 3: 183-192 (1980).
Foidart, J.M.; Foidart. J.B.: Van Cauwenberge. D.: Decaux, M.: Stul. M.; Lenaers, A.; Niebes. P.: Mahieu. P. R.: Synthesisoftypes I, III. IV and V (A. B) collagens by glomerular mesangial cells and aortic smooth muscle cells in culture: In Morel, Biochemistry of kidney functions. Inserm Symp., No.21, pp.23-34 (Elsevier. Amsterdam 1982).
Gay, S.: Martin, G. R.: Müller, P. K.; Timpl, R.; Kühn, K.: Simultaneous synthesis of types I and 111 collagen by fibroblasts in culture. Proc. natn. Acad. Sei. USA 73: 4037-4040 (1976).
Hassell. J R.: Robey. P.G.: Barrach, H.J.: Wilezek. J.: Rennard, S. I.: Martin. G. R.: Isolation ofa heparan sulfate-containing proteoglycan from basement membrane. Proc. natn. Acad. Sci. USA 77: 4494-4498 (1980).
Kanwar. Y.S.: Farquhar, M.G.: Presence of heparan sulfate in the glomerular basement membrane. Proc. natn. Acad. Sci. USA. 76: 1303-1307 (1979).
Mahieu. P. R.; Foidart. J. B.: Dubois, C. H.: Dechennc. CA.: Delieneffe. J.: Tissue culture of normal rat glomeruli. Contractile activity of tile cultivated mesangial cells. Invest. Cell Pathol. 3: 121-128 (1980).
Orkin. R.W.; Gehron, P,: McGoodwin, E.; Martin. G.R.; Valentine. T.: Swarm, R.: A murine tumor producing a matrix of basement membrane. J. exp. Med. 145: 204-220 (1977).
Scheinman. J.I.: Foidart. J.M.; Michael. A. F.: The immunohistology of glomerular antigens. V. The collagenous antigens of the glomerulus. Lab. Invest. 43: 373-381 (1980).
