[en] Laminin was demonstrated by immunoperoxidase and immunofluorescence staining in sections of normal human tissues fixed in formalin and routinely processed in paraffin. Exposure of the sections to a solution of pepsin (Burns et al. (1980) Histochemistry 67:73-78) revealed the antigenicity of this basement membrane glycoprotein. Sections from paraffin blocks stored for years at room temperature could be stained with this procedure. Normal human tissues, developing fetal tissues and tumors could be stained with this method. The staining patterns were similar to those seen in unfixed frozen sections. It thus appears that basement membrane components can be detected by immunohistological means from routinely processed histological samples, once the sections are pretreated with proteases. Staining for laminin could be used in embryonic studies and in histopathology to study the relation of cells to basement membranes and for the visualization of normal and abnormal vascularization.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ekblom, P.
Miettinen, M.
Rapola, J.
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Language :
English
Title :
Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues.
Bender B.L., Jaffe R., Carlin B., Chung A.E. (1981) Immunolocalization of entactin, a sulfated basement membrane component, in rodent tissues, and comparison with GP-2 (laminin). Am J Pathol 103:419-426.
DeLellis R.A., Sternberger L.A., Mann R.B., Banke P.M., Nakane B.K. (1979) Immunoperoxidase technique in diagnostic pathology. Am J Clin Pathol 71:483-488.
Dixon A.J., Burns J., Dunnhill, McGee J. (1980) Distribution of fibronectin in normal and diseased human kidneys. J Clin Pathol 33:1021-1028.
Ekblom P. (1981) Formation of basement membranes in the embryonic kidney. An immunohistological study. J Cell Biol 91:1-10.
Ekblom P., Alitalo K., Vaheri A., Timpl R., Saxén L. (1980) Induction of a basement membrane glycoprotein in embryonic kidney: possible role of laminin in morphogenesis. Proc Natl Acad Sci USA 77:485-469.
Flotte T.J., Bell D.A., Greco M.A. (1980) Tubular carcinoma and sclerosing adenosis. The use of basal lamina as a differential feature. Am J Surg Pathol 4:75-77.
Foidart J.M., Reddi A. (1980) Immunofluorescent localization of type IV collagen, and laminin during endochondral bone differentiation and regulation by pituitary growth hormone. Dev Biol 75:130-136.
Foidart J.M., Bere E.W., Yaar M., Rennard S., Gullino M., Martin G.R. (1980) Distribution and immunoelectron microscopic localization of laminin, a non-collagenous basement membrane glycoprotein. Lab Invest 42:336-342.
Franklin R.M., Martin M.T. (1981) Pre-embedding immunohistochemistry as an approach to high resolution antigen localization at the light microscopy level. Histochemistry 72:173-190.
Hahn E., Wick G., Pencev D., Timpl R. (1980) Distribution of basement membrane proteins in normal and fibrotic human liver: collagen type IV, laminin and fibronectin. Gut 21:63-71.
Hausen P., Dreyer C. (1981) The use of polyacrylamide as an embedding medium for immunohistochemical studies of embryonic tissues. Stain Technol 56:287-293.
Hølund B., Clausen P.P., Clemmensen J. (1981) The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue. Histochemistry 72:291-299.
Liotta L.A., Foidart J.M., Gehron Robey P., Martin G.R., Gullino P.M. (1979) Identification of micrometastasis of breast carcinomas by presence of basement membrane collagen. Lancet 2:146-147.
Pitelka D.R., Hamamoto S.T., Taggart B.N. (1980) Basal lamina and tissue recognition in malignant mammary tumors. Cancer Res 40:1600-1611.
Rohde H., Wick G., Timpl R. (1979) Immunochemical characterization of the basement membrane glycoprotein laminin. Eur J Biochem 102:195-201.
Sternberger L.A. (1979) The unlabelled antibody peroxidase-antiperoxidase (PAP) method. Immunocytochemistry , L.A., Sternberger, John Wiley & Sons, New York; 297-321.
Timpl R., Rohde H., Gehron Robey P., Rennard S.I., Foidart J.M., Martin G.R. (1979) Laminin — a glycoprotein from basement membranes. J Biol Chem 254:9933-9937.
Timpl R., Martin G.R. (1982) Components of basement membranes. Immunochemistry of the extracellular matrix, vol II: Applications, H., Furthmayr, CRC Press, Boca Raton, Florida; .
Vracko R. (1974) Basal lamina scaffold-anatomy and significance for maintenance of orderly tissue structure. Am J Pathol 77:314-338.
