No full text
Article (Scientific journals)
Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues.
Ekblom, P.; Miettinen, M.; Rapola, J. et al.
1982In Histochemistry, 75 (3), p. 301-7
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Abstract :
[en] Laminin was demonstrated by immunoperoxidase and immunofluorescence staining in sections of normal human tissues fixed in formalin and routinely processed in paraffin. Exposure of the sections to a solution of pepsin (Burns et al. (1980) Histochemistry 67:73-78) revealed the antigenicity of this basement membrane glycoprotein. Sections from paraffin blocks stored for years at room temperature could be stained with this procedure. Normal human tissues, developing fetal tissues and tumors could be stained with this method. The staining patterns were similar to those seen in unfixed frozen sections. It thus appears that basement membrane components can be detected by immunohistological means from routinely processed histological samples, once the sections are pretreated with proteases. Staining for laminin could be used in embryonic studies and in histopathology to study the relation of cells to basement membranes and for the visualization of normal and abnormal vascularization.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ekblom, P.
Miettinen, M.
Rapola, J.
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Language :
English
Title :
Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues.
Publication date :
1982
Journal title :
Histochemistry
ISSN :
0301-5564
Publisher :
Springer Verlag, Berlin, Germany
Volume :
75
Issue :
3
Pages :
301-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 February 2011

Statistics


Number of views
39 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
95
Scopus citations®
without self-citations
84
OpenCitations
 
88

Bibliography


Similar publications



Contact ORBi