[en] Twenty-five trabeculectomy specimens from patients with primary open angle glaucoma and chronic angle closure glaucoma, and 11 age-matched controls were examined by immunofluorescence and immunoperoxidase techniques to determine the types of collagen, immunoglobulins, and the presence of factor VIII-related antigen in the human aqueous drainage channels. In the glaucoma cases and in controls, we demonstrated that the electron dense basement membrane-like material in the peripheral portion of the trabecular beams and in the juxtacanalicular meshwork, consists at least in part, of type IV collagen, a noncollagenous protein ("laminin") and fibronectin. Factor VIII-related antigen was demonstrated in conjunctival vessels of the control eyes. Schlemm's canal and the trabecular endothelial cells did not stain for factor VIII-related/antigen in any of the specimens examined. No deposits of IgA, IgM, IgG, and the C3 component of complement were detected in the aqueous drainage channels.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Rodrigues, M. M.
Katz, S. I.
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Spaeth, G. L.
Language :
English
Title :
Collagen, factor VIII antigen, and immunoglobulins in the human aqueous drainage channels.
Rodrigues MM, Spaeth GL, Sivalingam E, Weinreb S (1976) Histopathology of 150 trabeculectomy specimens in glaucoma. Trans Ophthalmol Soc UK 96:245-255.
Rohen JW, Witmer R (1972) Electron microscopic studies on the trabecular meshwork in glaucoma simplex. Albrecht von Graefes Arch Klin Exp Ophthalmol 183:251-266.
Segawa K (1979) Electron microscopic changes of the trabecular tissue in primary open-angle glaucoma. Ann Ophthalmol 11:49-54.
Timpl R, Rohde H, Robey PG, Rennard SR, Foidart J, Martin GR (1979) Laminin-a glycoprotein from basement membrane. J Biol Chem 254:9933-9937.
Jaffe EA, Nachman RL (1975) Subunit of factor VIII antigen synthesized by human endothelial cells. J Clin Invest 56:698-702.
Becker B, Keates EU, Coleman SL (1975) Gamma globulin in the trabecular meshwork of glaucomatous eyes. Arch Ophthalmol 60:643-647.
Becker B, Unger HH, Coleman SL, Keatos EU (1963) Plasma cells and gamma globulin in trabecular meshwork of eyes with primary open-angle glaucoma. Invest Ophthalmol 70:38-41.
Shields MB, McCoy RC, Shelburne MD (1976) Immunofluorescent studies on trabecular meshwork in openangle glaucoma. Invest Ophthalmol 15:1014-1017.
Foidart J, Abe S, Martin GR (1978) Antibodies to type II collagen in relapsing polychondritis. N Engl J Med 299:1203-1207.
Engvall E, Rouslahti E (1977) Binding of soluble form of fibroblast surface protein, fibronectin to collagen. Int J Cancer 20:1-5.
.
Sternberger LA, Hardy PA, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase and anti-horseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315-333.
Holubar K, Wolff K, Donwad K, Beutner EG (1975) UItrastructural localization of immunoglobulins in bullous pemphigoid skin. Employment of a new peroxidase-antiperoxidase method. J Invest Dermatol 74:220-225.
Yaoita H, Gullino M, Katz SI (1976) Herpes gestationis. Ultra structure and ultrastructural localization of the in vivo-bound complement. J Invest Dermatol 66:383-388.
Prockop DJ, Kivirikko KI, Tuderman L, Guzman NA (1979) The biosynthesis of collagen and its disorders I. N Engl J Med 301:13-23.
Rohen JW (1979) Fine structure of trabecular meshwork in normal and glaucomatous eyes as seen in tangential section. Presented at the Annual Meeting of the Association for Research in Vision and Ophthalmology, Sarasota.
Harnisch JP (1976) Electron microscopic delineation of acid mucopolysaccharide in the trabecular meshwork. Klin Monats Augenheilk 169:90-94.
Segawa K (1975) Ultrastructural changes of the trabecular tissues in primary open-angle glaucoma. Jap J Ophthalmol 19:317-338.
Alvarado JA, Polansky JR (1979) Biologic activity of cultures of human trabecular cells. Presented at the Annual Meeting for Association of Research in Vision and Ophthalmology, Sarasota.
Spiro RG (1978) Nature of the glycoprotein components of basement membranes. Ann NY Acad Sci 312:106-121.
Bornstein P, Duksin D, Balian G, Davidson JM, Crouch E (1978) Organization of extracellular proteins on the connective tissue cell surface: Relevance to cell-matrix interactions in vitro and in vivo. Ann NY Acad Sci 312:93-105.
Jaffe EA, Mosher DF (1978) Synthesis of fibronectin by cultured human endothelial cells. Ann NY Acad Sci 312:122-131.
Lloyd C (1979) Fibronectin: a function at the junction. Nature 279:473-474.
Singer II (1979) The fibronexus: a transmembrane association of fibronectin containing fibers and bundles of 5 nm microfilaments in the hamster and human fibroblasts. Cell 16:675-678.
Gipson IK, Anderson RA (1979) Actin filaments in cells of human trabecular meshwork and Schlemm's canal. Invest Ophthalmol 18:547-561.
Foidart J (1979) Nature, localization and function of type IV collagen and laminin in basement membrane. Fed Proc 38:1047.
Allansmith MR, Whitney CR, McClellan BH, Newman LOP (1973) Immunoglobulins in the human eye location, type and amount. Arch Ophthalmol 89:36-45.
Thorgerisson G, Robertson AL (1978) The vascular endothelium-biologic significance. A review. Am J Pathol 93:805-848.
Johnston MC, Bhakdinaronk A, Reid YC (1974) An expanded role for the neural crest in oral and pharyngeal development. Fourth Symposium on Oral Sensation and Perceptions , JE Bosma, U.S. Government Printing Office, Washington, DC; 37-52.
Shields MB McCoy RC, Shelburne MD (1976) Immunofluorescent studies on trabecular meshwork in openangle glaucoma. Invest Ophthalmol 15:1014-1017.
Torczynski E, Jakobiec FA, Johnston MC, Font RL, Madewell JA (1977) Synophthalmia and cyclopia: a histopathologic, radiographic, and organogenetic analysis. Doc Ophthalmol 2:311-378.
