No full text
Article (Scientific journals)
Dynamics of mononuclear cadmium beta-lactamase revealed by the combination of NMR and PAC spectroscopy.
Hemmingsen, L.; Damblon, Christian; Antony, J. et al.
2001In Journal of the American Chemical Society, 123 (42), p. 10329-35
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Bacillus cereus/enzymology; Binding Sites; Binding, Competitive; Cadmium/chemistry/metabolism; Chelating Agents/chemistry/metabolism; Fluorescent Dyes/chemistry/metabolism; Fura-2/analogs & derivatives/chemistry/metabolism; Kinetics; Metalloproteins/chemistry/metabolism; Nuclear Magnetic Resonance, Biomolecular; Spectrometry, Gamma; beta-Lactamases/chemistry/metabolism
Abstract :
[en] The two metal sites in cadmium substituted beta-lactamase from Bacillus cereus 569/H/9 have been studied by NMR spectroscopy ((1)H, (15)N, and (113)Cd) and PAC spectroscopy ((111m)Cd). Distinct NMR signals from the backbone amides are identified for the apoenzyme and the mononuclear and binuclear cadmium enzymes. For the binuclear cadmium enzyme, two (113)Cd NMR signals (142 and 262 ppm) and two (111m)Cd PAC nuclear quadrupole interactions are observed. Two nuclear quadrupole interactions are also observed, with approximately equal occupancy, in the PAC spectra at cadmium/enzyme ratios < 1; these are different from those derived for the binuclear cadmium enzyme, demonstrating interaction between the two metal ion binding sites. In contrast to the observation from PAC spectroscopy, only one (113)Cd NMR signal (176 ppm) is observed at cadmium/enzyme ratios < 1. The titration of the metal site imidazole (N)H proton signals as a function of cadmium ion-to-enzyme ratio shows that signals characteristic for the binuclear cadmium enzyme appear when the cadmium ion-to-enzyme ratio is between 1 and 2, whereas no signals are observed at stoichiometries less than 1. The simplest explanation consistent with all data is that, at cadmium/enzyme ratios < 1, the single Cd(II) is undergoing exchange between the two metal sites on the enzyme. This exchange must be fast on the (113)Cd NMR time scale and slow on the (111m)Cd PAC time scale and must thus occur in a time regime between 0.1 and 10 micros.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Hemmingsen, L.
Damblon, Christian ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique structurale
Antony, J.
Jensen, M.
Adolph, H. W.
Wommer, S.
Roberts, G. C.
Bauer, R.
Language :
English
Title :
Dynamics of mononuclear cadmium beta-lactamase revealed by the combination of NMR and PAC spectroscopy.
Publication date :
2001
Journal title :
Journal of the American Chemical Society
ISSN :
0002-7863
eISSN :
1520-5126
Publisher :
American Chemical Society, Washington, United States - District of Columbia
Volume :
123
Issue :
42
Pages :
10329-35
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 November 2010

Statistics


Number of views
36 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
48
Scopus citations®
without self-citations
27
OpenCitations
 
51

Bibliography


Similar publications



Contact ORBi