Reference : A novel mitogen-inducible gene product related to p50/p105-NF-kappa B participates in...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
A novel mitogen-inducible gene product related to p50/p105-NF-kappa B participates in transactivation through a kappa B site.
Bours, Vincent mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique humaine >]
Burd, P. R. [> > > >]
Brown, K. [> > > >]
Villalobos, J. [> > > >]
Park, S. [> > > >]
Ryseck, R. P. [> > > >]
Bravo, R. [> > > >]
Kelly, K. [> > > >]
Siebenlist, U. [> > > >]
Molecular and Cellular Biology
American Society for Microbiology (ASM)
Yes (verified by ORBi)
[en] Amino Acid Sequence ; Ankyrins ; Base Sequence ; Blood Proteins/genetics ; Cloning, Molecular ; Gene Expression Regulation/drug effects ; Humans ; Membrane Proteins/genetics ; Mitogens/pharmacology ; Molecular Sequence Data ; NF-kappa B/genetics/metabolism ; Oncogene Proteins v-rel ; Open Reading Frames/genetics ; Phosphoproteins/genetics ; Precipitin Tests ; Protein Precursors/genetics/metabolism ; Protein-Tyrosine Kinases/genetics ; Repetitive Sequences, Nucleic Acid/genetics ; Retroviridae Proteins, Oncogenic/genetics ; T-Lymphocytes/metabolism ; Transcription Factors/genetics ; Transcriptional Activation/genetics ; Tumor Cells, Cultured
[en] A Rel-related, mitogen-inducible, kappa B-binding protein has been cloned as an immediate-early activation gene of human peripheral blood T cells. The cDNA has an open reading frame of 900 amino acids capable of encoding a 97-kDa protein. This protein is most similar to the 105-kDa precursor polypeptide of p50-NF-kappa B. Like the 105-kDa precursor, it contains an amino-terminal Rel-related domain of about 300 amino acids and a carboxy-terminal domain containing six full cell cycle or ankyrin repeats. In vitro-translated proteins, truncated downstream of the Rel domain and excluding the repeats, bind kappa B sites. We refer to the kappa B-binding, truncated protein as p50B by analogy with p50-NF-kappa B and to the full-length protein as p97. p50B is able to form heteromeric kappa B-binding complexes with RelB, as well as with p65 and p50, the two subunits of NF-kappa B. Transient-transfection experiments in embryonal carcinoma cells demonstrate a functional cooperation between p50B and RelB or p65 in transactivation of a reporter plasmid dependent on a kappa B site. The data imply the existence of a complex family of NF-kappa B-like transcription factors.

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