[en] Major histocompatibility (MHC) class I gene expression is synergistically induced by the cytokines TNF-alpha and IFN-gamma. However, the mechanism that results in synergistic activation of these genes has remained unclear. We demonstrated here that TNF-alpha induced binding of NF kappa B p50 and p65 to the NF kappa B-like element of the MHC class I promoter termed region I and IFN-gamma induced binding of IRF-1 to the adjacent interferon consensus sequence (ICS). We further demonstrated that NF kappa B and IRF-1 physically interacted with each other and cooperatively induced MHC class I gene expression when cotransfected into CHP-126 neuroblastomas. These results provide a molecular mechanism by which TNF-alpha and IFN-gamma synergistically induce the expression of a variety of genes involved in immune responses, including MHC class I.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Drew, P. D.
Franzoso, G.
Becker, K. G.
Bours, Vincent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique humaine
Carlson, L. M.
Siebenlist, U.
Ozato, K.
Language :
English
Title :
NF kappa B and interferon regulatory factor 1 physically interact and synergistically induce major histocompatibility class I gene expression.
Publication date :
1995
Journal title :
Journal of Interferon and Cytokine Research
ISSN :
1079-9907
eISSN :
1557-7465
Publisher :
Mary Ann Liebert, Inc., Larchmont, United States - New York
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