Trypsin and Trypsinogen from an Antarctic Fish: Molecular Basis of Cold Adaptation

Genicot, S.; Rentier-Delrue, Françoise; Edwards, D.; VanBeeumen, J.; Gerday, Charles

doi:10.1016/S0167-4838(96)00095-7

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Trypsin and Trypsinogen from an Antarctic Fish: Molecular Basis of Cold Adaptation

Genicot, S.; Rentier-Delrue, Françoise; Edwards, D. et al.

1996 • In Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, 1298 (1), p. 45-57

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https://hdl.handle.net/2268/73164

DOI
10.1016/S0167-4838(96)00095-7

PubMed
8948488

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Keywords :

trypsin; cold adaptation; 3-D structure

Abstract :

[en] Trypsin from Antarctic fish Paranotothenia magellanica displays molecular and kinetic properties typical of enzymes produced by psychrophilic organisms. The enzyme has a high catalytic efficiency at low and moderate temperatures and is rapidly inactivated at temperatures higher than 30 degrees C. The nucleotide sequence was determined after mRNA extraction and cDNA synthesis. The cDNA encodes a pretrypsinogen which includes a seven residue activation peptide containing only three acidic residues preceeding the 222 amino-acid mature enzyme. A three-dimensional model of the enzyme was built. Structural parameters possibly involved in the adaptation to cold have been derived from comparison with the three-dimensional structure of the bovine enzyme. Among them are the lack of Tyr-151 in the substrate binding pocket, an overall decrease in the number of salt bridges and hydrophobicity and the increase in the surface hydrophilicity.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Genicot, S.

Rentier-Delrue, Françoise ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire

Edwards, D.

VanBeeumen, J.

Gerday, Charles ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)

Language :

English

Title :

Trypsin and Trypsinogen from an Antarctic Fish: Molecular Basis of Cold Adaptation

Publication date :

14 November 1996

Journal title :

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

ISSN :

0167-4838

Publisher :

Elsevier, Netherlands

Volume :

1298

Issue :

Pages :

45-57

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 01 October 2010

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