[en] This study was carried out to investigate stress induced in a cross-country by measuring plasma cortisol and to compare the changes in cortisol level with the leucocytic changes measured with the quantitative buffy coat (QBC) analysis, which is a new method applicable in field conditions for the evaluation of haematological parameters in horses. Seven healthy horses competing in a national three-day event were investigated. Venous blood was sampled under resting condition, 2 min and 180 min after the completion of the cross-country and analysed for haematological parameters, plasma cortisol, protein and lactate levels. Immediately after the event, there was a significant increase in all values except in the granulocytes (Gr) to lymphocytes-monocytes (LM) ratio. 180 min after the event, all the parameters returned to their resting levels while white blood cells (WBC), Gr and Gr/LM ratio were significantly higher with respect to their resting values. A high correlation (r = 0.82) was found between the relative increase in plasma cortisol levels 2 min after exercise and the relative increase in Gr/LM 180 min after exercise. Therefore it was concluded that the Gr/LM ratio measured 180 min after exercise with the QBC analysis is a reliable index to estimate the plasma cortisol levels measured 2 min after exercise. This new technique could consequently be used in order to evaluate, in field conditions, the exercise-induced stress.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Linden, Annick ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires
Art, Tatiana ; Université de Liège - ULiège > Département de sciences fonctionnelles > Phys. neuro-muscul., de l'effort - Méd. sport. des animaux
Amory, Hélène ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés
Massart, A. M.
Burvenich, C.
Lekeux, Pierre ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
Language :
English
Title :
Quantitative Buffy Coat Analysis Related to Adrenocortical Function in Horses During a Three-Day Event Competition
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Bibliography
Alam M.G.S., Dobson P. (1986) Effect of various veterinary procedures on plasma concentrations of Cortisol, luteinising hormone and prostaglandin F2a metabolite in the cow. Vet. Rec. 118:7-10.
Art T., Amory H., Desmecht D., Lekeux P. (1990) Effects of show jumping on heart rate, blood lactate and other plasma values. Eq. Vet. J. 22:78-82.
Becker B.A., Nienaber J.A., Christenson R.K., Manak R.C., de Shzer J.A., Hahn G.L. (1985) Peripheral concentrations of Cortisol as an indicator of stress in the pig. Am. J. Vet. Res. 46:1034-1038.
Bottoms G.D., Roesel O.F., Rausch F.D., Akins E.L. (1972) Circadian variation in plasma Cortisol and corticosterone in pigs and mares. Am. J. Vet. Res. 33:785-790.
Burguez P.N., Ousey J., Cash R.S.G., Rossdale P.D. (1983) Changes in blood neutrophil and lymphocyte counts following administration of Cortisol to horses and foals. Eq. Vet. J. 15:58-60.
Carlson G.P. (1987) Hematology and Body Fluids in the Equine Athlete: a Review. Equine Exercise Physiology 2 , Gillespie, J. R., N. E. Robinson, ICEEP Publications, Davis, California, pp.; 393-425.
Dybdal N.O., Gribble D., Madigan J.E., Stabenfeldt G.H. (1980) Alterations in plasma corticosteroids, insulin and selected metabolites in horses used in endurance rides. Eq. Vet. J. 12:137-140.
Fulkerson W.J., Jamieson P.A. (1982) Pattern of Cortisol release in sheep following administration of synthetic ACTH or imposition of various stressor agents. Austr. J. Biol. Sci. 35:215-222.
Garrey W.E., Butler V. (1929) Physiological leukocytosis. Am. J. Physiol. 90:355-356.
Holson R.R., Scallet A.C., Ali S.F., Sullivan P., Gough B. (1988) Adrenocortical, B‐endorphin and behavioral responses to graded stressors in differentially reared rats. Physiol. Behav. 42:125-130.
Hoffsis G.F., Murdick P.W., Tharp V.L., Ault K. (1970) Plasma concentrations of Cortisol and corticosterone in the normal horse. Am. J. Vet. Res. 31:1379-1387.
Larsson M., Edqvist L.‐E., Ekman L., Persson S. (1979) Plasma Cortisol in the horse, diurnal rhythm and effects of exogenous ACTH. Acta Vet. Scand. 20:16-24.
Levine R.A., Hart A.H., Wardlaw S.C. (1986) Quantitative buffy coat analysis of blood collected from dogs, cats, and horses. J. Am. Vet. Med. Assoc. 189:670-673.
Linden A., Art T., Amory H., Desmecht D., Rollin F., Leroy P., Massart A.‐M., Burvenich C., Lekeux P., Changes in plasma Cortisol and lactate concentrations associated with 5 competitive sports in horses. Res. Vet. Sci. submitted for publication.; 1990.
Martinez R., Godoy A., Naretto E., White A. (1988) Neuroendocrine changes produced by competition stress on the thoroughbred race horse. Comp. Biochem. Physiol. 91 A:599-602.
Persson S.G.B. (1967) On blood volume and working capacity in horses. Acta Vet. Scandin. 19:1-189.
Persson S.G.B., Larsson M., Lindholm A. (1980) Effects of training on adrenocortical function and red‐cell volume in trotters. Zbl. Vet. Med. A 27:261-268.
Persson S.G.B. (1983) Evaluation of exercise tolerance and fitness in the performance horse. Equine Exercise Physiology , Snow, D. H., S. G. B. Persson, R. J. Rose, Granta Editions; 441-457.
Ralston J.M., Stenhouse A.M., Stenhouse N.S., Buck G.J., Lucks S.F., Reynoldson J.A., Bolton J.R. (1988) Cortisol concentrations in blood and urine of horses. Austr. Vet. J. 65:1-5.
Revington M. (1983) Haematology of the racing thoroughbred in Australia 1: reference values and the effect of excitement. Eq. Vet. J. 15:141-144.
Rose R.J., Ilkiw J.E., Arnold K.S., Backhouse J.W., Sampson D. (1980) Plasma biochemistry in the horse during 3‐day event competition. Eq. Vet. J. 12:132-136.
Rose R.J., Hodgson D.R. (1982) Haematological and plasma biochemical parameters in endurance horses during training. Eq. Vet. J. 14:144-148.
Rossdale P.D., Burguez P.N., Cash R.S.G. (1982) Changes in blood neutrophil/lymphocyte ratio related to adrenocortical function in the horse. Eq. Vet. J. 14:293-298.
Snow D.H., Mackenzie G. (1977) Effect of training on some metabolic changes associated with submaximal endurance exercise in the horse. Eq. Vet. J. 9:226-230.
Snow D.H., Rose R.J. (1981) Hormonal changes associated with long distance exercise. Eq. Vet. J. 13:195-197.
Snow D.H., Mason D.K., Ricketts S.W., Douglas T.A. (1983) Post‐race blood biochemistry in thoroughbreds. Equine Exercise Physiology , Snow, D. H., S. G. B. Persson, R. J. Rose, Granta Editions; 389-399.
Snow D.H., Rickett S.W., Mason D.K. (1983) Haematological response to racing and training exercise in thoroughbred horses, with particular reference to the leucocyte response. Eq. Vet. J. 15:149-154.
Valberg S., Gustavsson B.E., Lindholm A., Persson S.G.B. (1989) Blood chemistry and skeletal muscle metabolic responses during and after different speeds and durations of trotting. Eq. Vet. J. 21:91-95.
Wardlaw S.C., Levine R.A. (1983) Quantitative buffy coat analysis. A new laboratory tool functioning as a screening complete blood cell count. J. Am. Med. Assoc. 249:617-620.
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